anti-Human RAF1 antibody for Enzyme Immunoassay

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • RAF proto-oncogene serine/threonine-protein kinase pseudogene
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • LOC100341921
  • raf1.S
Epitope
N-Term
79
72
47
46
46
43
41
34
34
28
27
21
21
19
18
16
15
15
15
15
15
12
7
7
7
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
829
408
385
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Rabbit
774
63
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
30
30
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
506
365
218
172
156
103
50
49
20
18
17
7
2
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN360145
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358264 EIA Rabbit Ig Fraction pSer296 Log in to see Polyclonal 5
1 ABIN358300 EIA Rabbit Ig Fraction Ser43, pSer43 Log in to see Polyclonal 5
1 ABIN358302 EIA Rabbit Ig Fraction pSer471 Log in to see Polyclonal 5
1 ABIN358313 EIA Rabbit Ig Fraction pThr269 Log in to see Polyclonal 2
1 ABIN358317 EIA WB Rabbit Ig Fraction pSer338 Log in to see Polyclonal 2
1 ABIN358378 EIA Rabbit Ig Fraction pSer259 Log in to see Polyclonal 5
1 ABIN358380 EIA Rabbit Ig Fraction pTyr340 Log in to see Polyclonal 5
1 ABIN358392 DB EIA Rabbit Ig Fraction pSer494 Log in to see Polyclonal 5
1 ABIN358407 EIA Rabbit Ig Fraction pTyr341 Log in to see Polyclonal 5
1 ABIN358442 EIA Rabbit Ig Fraction pSer339 Log in to see Polyclonal 2
1 ABIN5551093 EIA WB Chicken N-Term Log in to see Polyclonal
1 ABIN1108823 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 4G4 2
1 ABIN126881 EIA WB Mouse IgG2a pSer621 Log in to see 6B4
1 ABIN114553 EIA WB Mouse IgG1 Log in to see 410
1 ABIN452646 EIA WB Mouse IgG1 N-Term Log in to see 540
1 ABIN452648 EIA WB Mouse IgG2b N-Term Log in to see 163
1 ABIN452647 EIA WB Mouse IgG1 N-Term Log in to see 863

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope N-Term
(79), (72), (47), (46), (46), (43), (41), (34), (34), (28), (27), (21), (21), (19), (18), (16), (15), (15), (15), (15), (15), (12), (7), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(829), (408), (385), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(774), (63), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(45), (45), (44), (30), (30), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(506), (365), (218), (172), (156), (103), (50), (49), (20), (18), (17), (7), (2), (2), (2), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN360145) Images
Specificity This antibody reacts to RAF1.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human RAF1.
Isotype Ig Fraction

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 antibody (ABIN360145) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID 5894, 9606
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 antibody (ABIN360145) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 antibody (ABIN360145) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-RAF1 antibody (ABIN360145)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Chong, Guan: "Regulation of Raf through phosphorylation and N terminus-C terminus interaction." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36269-76, 2003

Lee, Koh, Han: "Down-regulation of Raf-1 kinase is associated with paclitaxel resistance in human breast cancer MCF-7/Adr cells." in: Cancer letters, Vol. 193, Issue 1, pp. 57-64, 2003

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN360145) back to top
Supplier Images
 image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (N-Term) antibody (ABIN360145) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (N-Term) antibody
 image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (N-Term) antibody (ABIN360145) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (N-Term) antibody (Image 2)