anti-Human RAF1 antibody for Immunocytochemistry

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • raf1.S
Epitope
pSer259
84
75
48
48
47
39
34
34
34
31
31
22
20
20
19
19
16
15
15
15
14
14
10
10
10
8
8
7
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
873
456
427
25
23
17
16
16
8
8
8
5
4
4
2
2
1
1
Host
Rabbit
839
45
11
5
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
549
404
252
182
156
108
60
35
29
18
16
10
2
2
2
1
Supplier
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Catalog No. ABIN6256220
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16 ABIN6256385 ELISA ICC IF IHC WB Rabbit IgG pSer621 Log in to see Polyclonal 0
16 ABIN6260343 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
14.5 ABIN6255230 ELISA ICC IF WB Rabbit IgG pSer338 Log in to see Polyclonal 2
12.576219 ABIN5618996 ICC IF WB Rabbit Center Log in to see Polyclonal 0
11.5 ABIN6260344 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 2
10 ABIN6256250 ELISA ICC IF IHC WB Rabbit IgG pSer296 Log in to see Polyclonal 0
7 ABIN6254887 ELISA ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN6255183 ELISA ICC IF WB Rabbit IgG pSer301 Log in to see Polyclonal 0
7 ABIN6271606 ELISA ICC IF WB Rabbit IgG pTyr340 Log in to see Polyclonal 0
7 ABIN6260342 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2627495 ICC IF WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2857837 ICC IF IHC WB Rabbit pSer621 Log in to see Polyclonal 0
1 ABIN2858758 ICC IF IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer259
(84), (75), (48), (48), (47), (39), (34), (34), (34), (31), (31), (22), (20), (20), (19), (19), (16), (15), (15), (15), (14), (14), (10), (10), (10), (8), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(873), (456), (427), (25), (23), (17), (16), (16), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1)
Host Rabbit
(839), (45), (11), (5), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(45), (45), (44), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(549), (404), (252), (182), (156), (108), (60), (35), (29), (18), (16), (10), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN6256220) Images
Specificity Phospho-C-RAF (Ser259) Antibody detects endogenous levels of C-RAF only when phosphorylated at Serine 259
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human C-RAF around the phosphorylation site of Serine 259
Isotype IgG
Plasmids, Primers & others

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 antibody (ABIN6256220) Images back to top
Antigen
Alternative Name C-RAF (RAF1 Antibody Abstract)
Background

Description: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Gene: RAF1

Molecular Weight 74kDa
Gene ID 5894
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 antibody (ABIN6256220) Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 antibody (ABIN6256220) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-RAF1 antibody (ABIN6256220)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Cui, Lin, Shi, Pan: "Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway." in: Biochemical and biophysical research communications, Vol. 491, Issue 2, pp. 277-284, 2017

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN6256220) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody (ABIN6256220) Western blot analysis of Phospho-C-RAF (Ser259) expression in various lysates
Immunofluorescence (fixed cells) (IF/ICC) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody (ABIN6256220) ABIN6267281 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Immunohistochemistry (IHC) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody (ABIN6256220) ABIN6267281 at 1/200 staining human colon carcinoma sections by IHC-P. The tissue was...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody (ABIN6256220) Western blot analysis of C-RAF phosphorylation expression in K562 whole cell lysates,...