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General |
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Antigen | V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies |
Epitope | N-Term Alternatives |
Reactivity | Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Chicken Alternatives |
Clonality | |
Conjugate | This RAF1 antibody is un-conjugated Alternatives |
Application |
Enzyme Immunoassay (EIA), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-RAF1 AntibodyTarget Details RAF1 Application Details Handling Images |
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Cross-Reactivity (Details) | Species reactivity (tested):Human, mouse, rat |
Purification | Affinity chromatography purified via peptide column |
Immunogen | 19 amino acid peptide near the amino terminus of human C-raf |
Target Details RAF1Product Details anti-RAF1 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | RAF1 (RAF1 Antibody Abstract) |
Background | C-raf is the cellular homolog of viral raf gene (v-raf) and encodes a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases. Once activated, C-raf phosphorylates and activates the protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine specific protein kinases, ERK1 and ERK2. These activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf |
Gene ID | 5894 |
NCBI Accession | NP_002871 |
UniProt | P04049 |
Pathways | MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling |
Application DetailsProduct Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top |
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Application Notes | Optimal working dilution should be determined by the investigator. |
Restrictions | For Research Use only |
HandlingProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top |
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Concentration | 1.0 mg/mL |
Buffer | PBS containing 0.02 % sodium azide |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | -20 °C |
Storage Comment | Store the antibody (in aliquots) at -20 °C. |