anti-Mouse (Murine) RPS6KA1 antibody for Immunofluorescence (Cultured Cells)

Recommended RPS6KA1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
  • Rsk1
  • p90rsk
  • rsk
  • HU-1
  • MAPKAPK1A
  • RSK
  • RSK1
  • RPS6KA
  • hu-1
  • mapkapk1a
  • rsk1
  • CLS
  • HU-3
  • ISPK-1
  • MAPKAPK1B
  • MRX19
  • RSK2
  • S6K-alpha3
  • p90-RSK2
  • pp90RSK2
  • ribosomal protein S6 kinase polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Rps6ka1
  • RPS6KA1
  • rps6ka1
  • RPS6KA3
Epitope
pSer363, pThr359
58
53
37
28
27
25
14
13
12
10
10
9
9
8
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
352
184
152
35
16
10
9
6
6
4
4
2
2
1
1
1
1
Host
Rabbit
349
42
Clonality
Monoclonal
Conjugate
This RPS6KA1 antibody is un-conjugated
21
20
18
12
12
12
6
6
5
5
5
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Western Blotting (WB)
283
162
97
62
62
56
46
42
19
6
2
1
1
1
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Catalog No. ABIN5557523
$ 401.50
Plus shipping costs $45.00

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
Epitope pSer363, pThr359
(58), (53), (37), (28), (27), (25), (14), (13), (12), (10), (10), (9), (9), (8), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(352), (184), (152), (35), (16), (10), (9), (6), (6), (4), (4), (2), (2), (1), (1), (1), (1)
Host Rabbit
(349), (42)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RPS6KA1 antibody is un-conjugated
(21), (20), (18), (12), (12), (12), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Western Blotting (WB)
(283), (162), (97), (62), (62), (56), (46), (42), (19), (6), (2), (1), (1), (1)
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Product Details anti-RPS6KA1 Antibody

Target Details RPS6KA1 Application Details Handling Images
Purification Purified by Protein A.
Immunogen Synthetic peptide derived from human RSK1 p90(T359+S363), around 300-400aa (phospho T359+S363).
Clone 8H2
Isotype IgG

Target Details RPS6KA1

Product Details anti-RPS6KA1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RSK1 p90 (RPS6KA1 Antibody Abstract)
Background

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4).

Synonyms: p90 RSK1, p90rsk, p90S6K, pp90RSK1, Ribosomal protein S6 kinase 90kD 1, Ribosomal protein S6 kinase 90kD polypeptide 1, Ribosomal protein S6 kinase 90 kDa polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase polypeptide 1, Ribosomal S6 kinase 1, RPS6K1 alpha, rps6ka, Rps6ka1, RSK 1, RSK 1 p90, RSK, RSK1, S6K alpha 1, 90 kDa ribosomal protein S6 kinase 1, dJ590P13.1 ribosomal protein S6 kinase, 90kD, polypeptide 1, dJ590P13.1, EC 2.7.11.1, HU 1, HU1, MAP kinase activated protein kinase 1a, MAPKAP kinase 1A, MAPKAPK1A, MGC79981, Mitogen-activated protein kinase-activated protein kinase 1A

Gene ID 6195
UniProt Q15418
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling Images back to top
Application Notes WB 1:1000-2000
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer363), (pThr359) antibody (ABIN5557523) Lane 1: A431, Lane 2: 293, Lane 3: MCF-7 cell lysates probed with RSK1 p90(T359+S363)...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer363), (pThr359) antibody (ABIN5557523) A431 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS a...