anti-Human SIRT1 antibody for Western Blotting

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • sirtuin 1 L homeolog
  • SIRT1
  • SRT1
  • Sirt1
  • sirt1.L
Epitope
N-Term
26
24
21
17
15
15
11
11
10
6
6
5
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
300
139
68
21
14
10
6
6
6
6
5
3
3
2
2
2
2
1
Host
Rabbit
200
86
28
6
3
Clonality
Polyclonal
Conjugate
This SIRT1 antibody is un-conjugated
16
14
9
7
7
6
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Western Blotting (WB)
254
139
84
66
60
51
42
40
33
7
6
4
4
2
2
2
2
2
1
1
Options
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Pubmed
6 references available

Available images

Western Blotting (WB) image for anti-Sirtuin 1 (SIRT1) (N-Term) antibody (ABIN2779607)
Catalog No. ABIN2779607
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2286417 ABIN2779608 WB Rabbit N-Term Log in to see Polyclonal 3
3.2286417 ABIN2668278 ICC IF IP WB Mouse IgG1 AA 581-630 Log in to see 2G1-F7 2
3.2286417 ABIN729853 IF (p) IHC (p) WB Rabbit IgG AA 192-242 Log in to see Polyclonal 3
3.2286417 ABIN3042483 WB Rabbit IgG AA 112-311 Log in to see Polyclonal 0
3.2286417 ABIN4353901 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F3 1
3.2286417 ABIN2464533 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.2286417 ABIN1525672 IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.2286417 ABIN969396 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 1F3 2
3.2286417 ABIN151572 IP PLA WB Rabbit C-Term Log in to see Polyclonal 0
3.2286417 ABIN1874784 ChIP IF IHC IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.2286417 ABIN565071 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 456-555, partial Log in to see 7B7 3
3.2286417 ABIN4353905 SimWes WB Rabbit Log in to see Polyclonal 2
3.2286417 ABIN614713 EIA IF IHC (p) WB Mouse IgG1 AA 456-555 Log in to see 7B7 0
3.2286417 ABIN1882053 WB Mouse IgG3, kappa AA 1-180 Log in to see 1089CT5-3-1 5
3.2286417 ABIN4899450 CyTOF FACS WB Sheep IgG AA 2-747 Log in to see Polyclonal 0
3.2286417 ABIN3186962 ELISA IHC WB Rabbit IgG Tyr631 Log in to see Polyclonal 0
3.2286417 ABIN1176381 ICC IHC WB Rabbit IgG AA 244-498 Log in to see Polyclonal 0
3.2286417 ABIN1874783 IHC IP WB Rabbit IgG Log in to see Polyclonal 0
3.2286417 ABIN396774 IF WB Mouse IgG1 kappa AA 456-555 Log in to see 7B7 0
3.2286417 ABIN2682598 IP WB Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope N-Term
(26), (24), (21), (17), (15), (15), (11), (11), (10), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(300), (139), (68), (21), (14), (10), (6), (6), (6), (6), (5), (3), (3), (2), (2), (2), (2), (1)
Host Rabbit
(200), (86), (28), (6), (3)
Clonality
Polyclonal
Conjugate This SIRT1 antibody is un-conjugated
(16), (14), (9), (7), (7), (6), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Application Western Blotting (WB)
(254), (139), (84), (66), (60), (51), (42), (40), (33), (7), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1)
Pubmed 6 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling References for anti-SIRT1 antibody (ABIN2779607) Images
Blocking Peptide Blocking peptide for this product available: ABIN983762
Sequence PETIPPPELD DMTLWQIVIN ILSEPPKRKK RKDINTIEDA VKLLQECKKI
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against SIRT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SIRT1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling References for anti-SIRT1 antibody (ABIN2779607) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined,
Alias Symbols: SIR2L1
Protein Interaction Partner: SIRT2, KAT5, IRS1, ING2, ING1, HSP90AA1, AR, EP300, CDK1, CCNB1, RPS19BP1, KAT8, CLOCK, IRS2, UBE2I, TP53, STK11, WRN, PPARGC1A, FOXO1, VHL, UBC, E2F1, PSME3, KCNA5, KCNA4, KCNAB2, POP1, PPARA, CAP1, KIAA1598, TPM4, FASN, CDKL1, HIC1, HDAC4, CHD3, CDK6, S
Protein Size: 747
Molecular Weight 82 kDa
Gene ID 23411
NCBI Accession NM_012238, NP_036370
UniProt Q96EB6
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling References for anti-SIRT1 antibody (ABIN2779607) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 747 AA
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details References for anti-SIRT1 antibody (ABIN2779607) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SIRT1 antibody (ABIN2779607)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Takumida, Takumida, Katagiri, Anniko: "Localization of sirtuins (SIRT1-7) in the aged mouse inner ear." in: Acta oto-laryngologica, pp. 1-12, 2015

Cueno, Imai, Tamura, Ochiai: "Butyric acid-induced rat jugular blood cytosolic oxidative stress is associated with SIRT1 decrease." in: Cell stress & chaperones, Vol. 19, Issue 2, pp. 295-8, 2014

Hsu, Chuang: "Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera)." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 69, Issue 8, pp. 955-64, 2014

Hsu, Hu: "Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees." in: Biogerontology, Vol. 15, Issue 4, pp. 389-400, 2014

Anavekar, Mirza, Skali, Plappert, St John Sutton, Pfeffer, Solomon,: "Risk assessment in patients with depressed left ventricular function after myocardial infarction using the myocardial performance index--Survival and Ventricular Enlargement (SAVE) experience." in: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography, Vol. 19, Issue 1, pp. 28-33, 2006

Background publications

Solomon, Pasupuleti, Xu, McDonagh, Curtis, DiStefano, Huber: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, 2005

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling References for anti-SIRT1 antibody (ABIN2779607) back to top
Supplier Images
Western Blotting (WB) image for anti-Sirtuin 1 (SIRT1) (N-Term) antibody (ABIN2779607) anti-Sirtuin 1 (SIRT1) (N-Term) antibody