LMW DNA Ladder

Details for Product No. ABIN1540462, Supplier: Log in to see
Detection Range
25-700 bp
Minimum Detection Limit
25 bp
Agarose Gel Electrophoresis (AGE)
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Purpose LMW DNA Ladder is ideal for determining the size of double-stranded DNA from 25 to 700 base pairs.
Detection Method Fluorometric
Characteristics The ladder consists of 10 linear double-stranded DNA fragments, chromatography- purified. The intensity of the 100bp and 300bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 300bp are at 40ng. The 100bp and 300bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
10 DNA Fragments (bp): 25, 50, 75, 100, 150, 200, 300, 400, 500, and 700
100bp and 300bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 25bp-700bp
Bands: 10
Application Notes Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment 1 year at room temperature, for long term storage, please keep at -20°C.
Expiry Date 12 months
Supplier Images
Agarose Gel Electrophoresis (AGE) image for LMW DNA Ladder (ABIN1540462) LMW DNA Ladder