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- Detection Method
- Fluorometric
- Detection Range
- 25-700 bp
- Minimum Detection Limit
- 25 bp
- Application
- Agarose Gel Electrophoresis (AGE)
- Purpose
- LMW DNA Ladder is ideal for determining the size of double-stranded DNA from 25 to 700 base pairs.
- Characteristics
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The ladder consists of 10 linear double-stranded DNA fragments, chromatography- purified. The intensity of the 100bp and 300bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 300bp are at 40ng. The 100bp and 300bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
10 DNA Fragments (bp): 25, 50, 75, 100, 150, 200, 300, 400, 500, and 700
100bp and 300bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 25bp-700bp
Bands: 10
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Protein Molecular Weight Marker
14-120 kDa Colorimetric
BLUltra Prestained Protein Ladder6.5-270 kDa Colorimetric
100bp DNA Ladder100-1500 bp
OneMARK B DNA Ladder250-10000 bp
OneMARK 100 DNA Ladder100-3000 bp
50bp DNA Ladder50-1,500 bp Fluorometric
BlueAQUA Prestained Protein Ladder10-180 kDa Colorimetric
BlueRAY Prestained Protein Ladder10-180 kDa Colorimetric
BLUeye Prestained Protein Ladder10-245 kDa Colorimetric
BLUelf Prestained Protein Ladder5 kDa - 245 kDa Colorimetric
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- Application Notes
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Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h. - Comment
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Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I. - Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- -20 °C
- Storage Comment
- 1 year at room temperature, for long term storage, please keep at -20°C.
- Expiry Date
- 12 months
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