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BLUltra Prestained Protein Ladder

6.5-270 kDa Colorimetric
Catalog No. ABIN2868518
  • Detection Method
    Colorimetric
    Detection Range
    6.5-270 kDa
    Minimum Detection Limit
    6.5 kDa
    Application
    SDS-PAGE (SDS), Western Blotting (WB)
    Purpose
    The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa.
    Characteristics
    Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
  • Application Notes
    - 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
    - 2.5 μl per well for general Western transferring.
    Reagent Preparation

    Quality Control: Under suggested conditions, BLUltra Prestained Protein Ladder resolves 10 major bands in 4-12 % Bis-Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Storage Buffer: 62.5 mM Tris-H3PO4 ( pH 7.5 at 25 °C), 1 mM EDTA, 2 % SDS, 10 mM DTT, 1 mM Sodium azide, 33 % glycerol.
    Storage
    -20 °C
    Storage Comment
    Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C.For long term storage, store at -20°C.
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