We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) Antibodies|
|Epitope||AA 461-491, C-Term Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
Product detailsTarget Details Application Details Handling References Images
|Specificity||This antibody detects Aromatase (CYP19A1) at C-term.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS|
|Immunogen||KLH conjugated synthetic peptide between 461~491 amino acids from the C-terminal region of Human CYP19A1. Genename: CYP19A1|
Target DetailsProduct details Application Details Handling References Images back to top
|Alternative Name||Aromatase (CYP19A1 Antibody Abstract)|
|Background||CYP19A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in the gene encoding CYP19A1 can result in either increased or decreased aromatase activity, the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation.Synonyms: ARO1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P450 19A1, Estrogen synthetase, P450AROM|
|Molecular Weight||57883 Da|
|Gene ID||1588, 9606|
|Pathways||Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis|
Application DetailsProduct details Target Details Handling References Images back to top
ELISA: 1/1,000. Western blot: 1/50-1/100. Immunohistochemistry on Paraffin Sections: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct details Target Details Application Details References Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ReferencesProduct details Target Details Application Details Handling Images back to top
Xita, Georgiou, Lazaros, Psofaki, Kolios, Tsatsoulis: "The synergistic effect of sex hormone-binding globulin and aromatase genes on polycystic ovary syndrome phenotype." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 158, Issue 6, pp. 861-5, 2008
Meinsma, Fernandez-Salguero, Van Kuilenburg, Van Gennip, Gonzalez: "Human polymorphism in drug metabolism: mutation in the dihydropyrimidine dehydrogenase gene results in exon skipping and thymine uracilurea." in: DNA and cell biology, Vol. 14, Issue 1, pp. 1-6, 1995
ImagesProduct details Target Details Application Details Handling References back to top