anti-Horse (Equine) HSD3B1 antibody for Western Blotting

Recommended HSD3B1 Antibody (supplied by: Log in to see )

Antigen
Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1) Antibodies
  • 3BETAHSD
  • HSD3B
  • HSDB3
  • HSDB3A
  • I
  • SDR11E1
  • HSD3B1
  • MGC131206
  • D3Ertd383e
  • 3B-HSD
  • 3BHSD
  • si:rp71-68n21.10
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 L homeolog
  • HSD3B1
  • LOC469446
  • LOC713091
  • hsd3b1.L
  • LOC100451655
  • Hsd3b1
  • hsd3b1
Epitope
N-Term
12
12
12
11
7
4
2
1
1
Reactivity
Cow (Bovine), Goat, Horse (Equine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
91
21
20
15
15
5
1
1
Host
Rabbit
47
29
16
Clonality
Polyclonal
Conjugate
This HSD3B1 antibody is un-conjugated
6
6
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
74
44
29
21
20
20
17
3
Options
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Available images

Catalog No. ABIN2777011
$ 289.00
Plus shipping costs $45.00

General

Antigen Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1) Antibodies
Epitope N-Term
(12), (12), (12), (11), (7), (4), (2), (1), (1)
Reactivity Cow (Bovine), Goat, Horse (Equine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(91), (21), (20), (15), (15), (5), (1), (1)
Host Rabbit
(47), (29), (16)
Clonality
Polyclonal
Conjugate This HSD3B1 antibody is un-conjugated
(6), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(74), (44), (29), (21), (20), (20), (17), (3)
Pubmed 2 references available
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Product Details anti-HSD3B1 Antibody

Target Details HSD3B1 Application Details Handling References for anti-HSD3B1 antibody (ABIN2777011) Images
Blocking Peptide Blocking peptide for this product available: ABIN978268
Sequence TGWSCLVTGA GGFLGQRIIR LLVKEKELKE IRVLDKAFGP ELREEFSKLQ
Predicted Reactivity Cow: 79%, Goat: 82%, Horse: 79%, Human: 100%, Pig: 79%, Rat: 79%, Sheep: 79%
Characteristics This is a rabbit polyclonal antibody against HSD3B1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HSD3B1
Plasmids, Primers & others

Target Details HSD3B1

Product Details anti-HSD3B1 Antibody Application Details Handling References for anti-HSD3B1 antibody (ABIN2777011) Images back to top
Antigen
Alternative Name HSD3B1 (HSD3B1 Antibody Abstract)
Background 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Alias Symbols: HSD3B, HSDB3, I, HSDB3A, SDR11E1, 3BETAHSD
Protein Size: 373
Molecular Weight 42 kDa
Gene ID 3283
NCBI Accession NM_000862, NP_000853
UniProt P14060
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis

Application Details

Product Details anti-HSD3B1 Antibody Target Details HSD3B1 Handling References for anti-HSD3B1 antibody (ABIN2777011) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 373 AA

Restrictions For Research Use only

Handling

Product Details anti-HSD3B1 Antibody Target Details HSD3B1 Application Details References for anti-HSD3B1 antibody (ABIN2777011) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSD3B1 antibody (ABIN2777011)

Product Details anti-HSD3B1 Antibody Target Details HSD3B1 Application Details Handling Images back to top
Product cited in:

Mirkheshti, Park, Jiang, Cropper, Werner, Song, Chatterjee: "Dual targeting of androgen receptor and mTORC1 by salinomycin in prostate cancer." in: Oncotarget, Vol. 7, Issue 38, pp. 62240-62254, 2016

Background publications

Ross, Oh, Xie, Pomerantz, Nakabayashi, Sartor, Taplin, Regan, Kantoff, Freedman: "Inherited variation in the androgen pathway is associated with the efficacy of androgen-deprivation therapy in men with prostate cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 6, pp. 842-7, 2008

Images

Product Details anti-HSD3B1 Antibody Target Details HSD3B1 Application Details Handling References for anti-HSD3B1 antibody (ABIN2777011) back to top
Supplier Images
Western Blotting (WB) image for anti-Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1) (N-Term) antibody (ABIN2777011) Host: Rabbit Target Name: HSD3B1 Sample Type: Human Adult Placenta Antibody Dilution:...
Western Blotting (WB) image for anti-Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1) (N-Term) antibody (ABIN2777011) Lanes : Lane 1: 50ug monkey brain extract Primary Antibody Dilution : 1:1000 Seco...
Western Blotting (WB) image for anti-Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1) (N-Term) antibody (ABIN2777011) WB Suggested Anti-HSD3B1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positiv...