anti-Goat alpha Tubulin antibody for ELISA

Recommended alpha Tubulin Antibody

Antigen
alpha Tubulin (TUBA1) Antibodies
  • Tuba1
  • K-ALPHA-1
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • tuba1b
  • Tuba1b
Epitope
AA 427-441
32
28
17
12
11
9
8
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken, Cow (Bovine), Goat, Sheep (Ovine)
301
186
175
41
35
34
26
19
18
17
13
10
10
10
8
8
8
8
8
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
189
132
24
5
2
2
2
1
Clonality
Polyclonal
Conjugate
This alpha Tubulin antibody is conjugated to Biotin
21
10
8
8
8
8
8
8
8
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
270
110
103
91
73
72
55
31
18
16
13
8
3
2
1
1
1
Options
Catalog No. ABIN1043919
$ 493.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13.550404 ABIN964564 ELISA WB HRP Rabbit IgG AA 427-441 Polyclonal 0

General

Antigen alpha Tubulin (TUBA1) Antibodies
Epitope AA 427-441
(32), (28), (17), (12), (11), (9), (8), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chicken, Cow (Bovine), Goat, Sheep (Ovine)
(301), (186), (175), (41), (35), (34), (26), (19), (18), (17), (13), (10), (10), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(189), (132), (24), (5), (2), (2), (2), (1)
Clonality
Polyclonal
Conjugate This alpha Tubulin antibody is conjugated to Biotin
(21), (10), (8), (8), (8), (8), (8), (8), (8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(270), (110), (103), (91), (73), (72), (55), (31), (18), (16), (13), (8), (3), (2), (1), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-alpha Tubulin Antibody

Target Details alpha Tubulin Application Details Handling References for anti-alpha Tubulin antibody (ABIN1043919) Images
Specificity Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.  The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Characteristics Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Immunogen Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
Isotype IgG
Plasmids, Primers & others

Target Details alpha Tubulin

Product Details anti-alpha Tubulin Antibody Application Details Handling References for anti-alpha Tubulin antibody (ABIN1043919) Images back to top
Antigen
Alternative Name Alpha-Tubulin (TUBA1 Antibody Abstract)
Background Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.
Synonyms: Loading Control Antibody
Pathways Microtubule Dynamics

Application Details

Product Details anti-alpha Tubulin Antibody Target Details alpha Tubulin Handling References for anti-alpha Tubulin antibody (ABIN1043919) Images back to top
Application Notes Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
Comment

Gene Name: TUBA1B

Restrictions For Research Use only

Handling

Product Details anti-alpha Tubulin Antibody Target Details alpha Tubulin Application Details References for anti-alpha Tubulin antibody (ABIN1043919) Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 0.61 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-alpha Tubulin antibody (ABIN1043919)

Product Details anti-alpha Tubulin Antibody Target Details alpha Tubulin Application Details Handling Images back to top
Product cited in:

Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, 1985