|Antigen||Microtubule-Associated Protein 1B (MAP1B) Antibodies|
|Reactivity||Cow (Bovine), Chicken, Cat (Feline), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP1B antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
|1 reference available|
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Product Details anti-MAP1B AntibodyTarget Details MAP1B Application Details Handling References for anti-MAP1B antibody (ABIN269612) Images
|Specificity||The antibody does not cross-react with other MAPs or tubulin.|
|Immunogen||Rat brain microtubule associated proteins.|
Target Details MAP1BProduct Details anti-MAP1B Antibody Application Details Handling References for anti-MAP1B antibody (ABIN269612) Images back to top
|Alternative Name||MAP1B (MAP1B Antibody Abstract)|
|Background||Gene Symbol: MAP1B|
|Molecular Weight||Theoretical MW: 271 kDa|
|Pathways||Microtubule Dynamics, Regulation of Cell Size|
Application DetailsProduct Details anti-MAP1B Antibody Target Details MAP1B Handling References for anti-MAP1B antibody (ABIN269612) Images back to top
|Application Notes||Western Blot 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, MicroarrayThe observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for MAP1B Antibody IHC protocol for cryostat sections (Protocol generously provided by Dr. Yimin Wang, Tissue Regeneration Therapeutics Inc )
1. Take out the cryostat section slides from -80C. Dry for few minutes at RT.
. Rinse slides with PBS to get rid of OCT. 3 min x 2, shake
. Fix slides with 1:1 methanol:acetone for 3 min
. Wash slides with PBS, 3 min x
. Circle tissue sections with PAP-Pen,
. Incubation with Normal Horse Serum: 5 % in 2 %BSA (in PBS-Tween20) for 20 minutes.
. MAP1B anti human antibody (1:200 in 2 % BSA 0.1 % Tween20). Incubate for 1.5 hours at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %), 5min x2The following steps are for IHC only
. Blocking of endogenous peroxidase: Incubation with 0.3 % H2O2 for 10 minutes.
. Wash PBS-Tween (0.1 %), 3 min x
. Incubation with secondary antibody (1:200 with PBS, anti mouse-bio, in AK-5002 kit) for 45 minutes at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubate with VECTAINABC reagent solution (bottle C, Cat No: PK6100, contains Avidin-HRP) for 30 minutes at RT in the dark.(For solution C: 10ml PBS + 2 drops of A and 2 drops of B, kept in 4C with dark)Prewarm at RT for 10min before use and drop 2 drops to the sections.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubation with peroxidase substrate (Vector Nova Red, SK-4800) until desired stain intensity develops. Usually 1 to 2 minutes.Prepare the substrate immediately before use: can be used for > 1week.- Add 3 drops of Reagent 1 to 5 mL of DDW, mix well- Add 2 drops of Reagent 2 and mix well- Add 2 drops of Reagent 3 and mix well- Add 2 drops of Hydrogen Peroxide Solution and mix well
. Wash slides with tap water for 5 minutes.
. Hematoxylin nuclear staining: (Filter solution when first use, HE staining Jar,2nd one). Stain slides for 5 seconds at room temperature, then carefully rinse for 3 minutes under running cold tap water,
. Mount the slide with cc mount (Sigma, C9368, 30ml)
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP1B Antibody Target Details MAP1B Application Details References for anti-MAP1B antibody (ABIN269612) Images back to top
Buffer contains: 15 mM Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||Store at -20°C. Avoid freeze-thaw cycles.|
References for anti-MAP1B antibody (ABIN269612)Product Details anti-MAP1B Antibody Target Details MAP1B Application Details Handling Images back to top
|Product cited in:||
Kaneko, Arayatrakoollikit, Yamanaka, Ito, Okiji: "Immunohistochemical and gene expression analysis of stem-cell-associated markers in rat dental pulp." in: Cell and tissue research, Vol. 351, Issue 3, pp. 425-32, 2013 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry
ImagesProduct Details anti-MAP1B Antibody Target Details MAP1B Application Details Handling References for anti-MAP1B antibody (ABIN269612) back to top