anti-Plant TUBB antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended TUBB Antibody (supplied by: Log in to see )

Antigen
Tubulin, beta (TUBB) Antibodies
  • M40
  • OK/SW-cl.56
  • TUBB1
  • TUBB5
  • XLOT
  • m40
  • ok/sw-cl.56
  • tubb1
  • tubb5
  • TUBB2A
  • TUBB2B
  • TUBB
  • 1t
  • B1t
  • BETA 56D
  • CG9277
  • DTB2
  • Dmbeta1
  • Dmel\\CG9277
  • T
  • Tub
  • Tubulin
  • beta
  • beta-Tub
  • beta-Tub56D
  • beta-tub
  • beta-tubulin56D
  • beta1
  • beta1-Tubulin
  • beta1-tub
  • beta1Tub
  • beta1t
  • beta1tub
  • beta56D
  • betaTub
  • betaTub1
  • beta[[1]] tubulin
  • beta[[1]]-tubulin
  • betatub(56D)
  • 143391_i_at
  • 3t
  • B3t
  • BETA 60D
  • CG3401
  • D.m.BETA-60D
  • DTB3
  • Dmbeta3
  • Dmel\\CG3401
  • Tub60D
  • beta-Tub60D
  • beta-Tub6D
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3TUB
  • beta3Tub
  • beta3t
  • beta60C
  • betaTub3
  • betaTub60C
  • beta[[3]] tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • betatub60D
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • 2t
  • B2t
  • BETA 85D
  • BETA2
  • CG9359
  • D.m.BETA-85D
  • DTB4
  • Dmbeta2
  • Dmel\\CG9359
  • beta(2)Tu
  • beta(2)Tub
  • beta-Tub85D
  • beta-tub85D
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta85D
  • betaTub2
  • beta[[2]]-tubulin
  • ms(3)KK[D]
  • 4t
  • B4t
  • BETA 98B
  • CG4869
  • DTB1
  • Dmbeta4
  • Dmel\\CG4869
  • beta-Tub97EF
  • beta4
  • beta4t
  • beta97F
  • betaTub4
  • betaTub98BC
  • betaTub98C
  • Tub1
  • bmtub1
  • Tub3
  • bmtub3
  • Tub2
  • bmtub2
  • Tub4
  • bmtub4
  • LOC100101153
  • tubulin beta class I
  • putative cobalt-zinc-cadmium outer membrane resistance protein
  • Tubulin beta chain
  • tubulin beta-2A chain
  • beta-Tubulin at 56D
  • beta-Tubulin at 60D
  • beta-Tubulin at 85D
  • beta-Tubulin at 97EF
  • beta-tubulin
  • beta tubulin
  • tubulin beta class I L homeolog
  • tubulin, beta 5 class I
  • TUBB
  • czcC
  • tbb-6
  • tubb
  • LOC462399
  • betaTub56D
  • betaTub60D
  • betaTub85D
  • betaTub97EF
  • Tub1
  • Tub3
  • Tub2
  • Tub4
  • TVAG_525430
  • TVAG_062880
  • TVAG_456920
  • LOC100101153
  • Tb927.1.2330
  • Tb927.1.2350
  • Tb927.1.2370
  • Tb927.1.2390
  • tubb.L
  • Tubb
  • LOC693049
  • LOC443015
  • Tubb5
Epitope
N-Term
52
25
15
15
12
6
6
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine), Mouse (Murine), Plant
387
285
269
62
58
53
46
35
30
27
21
21
19
16
12
10
10
10
9
8
7
6
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
253
176
1
1
1
Clonality
Monoclonal
Conjugate
This TUBB antibody is un-conjugated
13
8
7
7
6
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
365
156
138
112
106
90
41
29
25
24
8
7
3
3
2
2
1
1
Options
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Catalog No. ABIN192340
$ 290.95
Plus shipping costs $45.00

General

Antigen Tubulin, beta (TUBB) Antibodies
Epitope N-Term
(52), (25), (15), (15), (12), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Pig (Porcine), Mouse (Murine), Plant
(387), (285), (269), (62), (58), (53), (46), (35), (30), (27), (21), (21), (19), (16), (12), (10), (10), (10), (9), (8), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(253), (176), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB antibody is un-conjugated
(13), (8), (7), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(365), (156), (138), (112), (106), (90), (41), (29), (25), (24), (8), (7), (3), (3), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-TUBB Antibody

Target Details TUBB Application Details Handling References for anti-TUBB antibody (ABIN192340) Images
Specificity The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.
Purification Purified from ascites by precipitation methods and size-exclusion chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen beta-tubulin from porcine brain
Clone TU-13
Isotype IgM
Plasmids, Primers & others

Target Details TUBB

Product Details anti-TUBB Antibody Application Details Handling References for anti-TUBB antibody (ABIN192340) Images back to top
Antigen
Alternative Name TUBB (TUBB Antibody Abstract)
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.
Pathways Microtubule Dynamics, M Phase

Application Details

Product Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB antibody (ABIN192340) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB antibody (ABIN192340) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-TUBB antibody (ABIN192340)

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top
Background publications

Linhartová, Dráberová, Viklický, Dráber: "Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells." in: FEBS letters, Vol. 320, Issue 1, pp. 79-82, 1993

Product cited in:

Dráber, Vater, Böhm, Kuklova, Unger: "Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies." in: FEBS letters, Vol. 262, Issue 2, pp. 209-11, 1990

Dráber, Lagunowich, Dráberová, Viklický, Damjanov: "Heterogeneity of tubulin epitopes in mouse fetal tissues." in: Histochemistry, Vol. 89, Issue 5, pp. 485-92, 1988

Images

Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling References for anti-TUBB antibody (ABIN192340) back to top
Supplier Images
Western Blotting (WB) image for anti-Tubulin, beta (TUBB) (N-Term) antibody (ABIN192340) Western blotting analysis of porcine brain lysate using anti-beta tubulin (TU-13) pur...