anti-Cat (Feline) Smooth Muscle Actin antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Smooth Muscle Actin Antibody

Antigen
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
  • ASMA
  • SMactin
  • a-sma
  • aat6
  • actsa
  • mymy5
  • sm-actin
  • ACTA2
  • actc1
  • ACT-4
  • AAT6
  • ACTSA
  • MYMY5
  • 0610041G09Rik
  • Actvs
  • SMalphaA
  • a-SMA
  • alphaSMA
  • wu:fb63d03
  • asma
  • actin, alpha 2, smooth muscle, aorta L homeolog
  • actin, alpha 2, smooth muscle, aorta
  • acta2.L
  • ACTA2
  • acta2
  • Acta2
Epitope
N-Term
149
5
4
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
401
261
204
85
72
67
58
42
32
30
29
29
27
26
13
7
4
3
2
1
1
1
Host
Mouse
236
156
16
Clonality
Monoclonal
Conjugate
This Smooth Muscle Actin antibody is conjugated to CF405M
17
7
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
208
162
129
117
97
95
63
21
19
17
14
6
4
3
3
2
1
1
1
Options
Catalog No. ABIN6155621
$ 231.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN6155643 FACS IF IHC (f) CF®594 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155627 FACS IF IHC (f) CF®647 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155637 FACS IF IHC (f) CF®680 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155635 FACS IF IHC (f) CF®770 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155619 FACS IF IHC (f) CF®405S Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155641 FACS IF IHC (f) CF®488A Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155629 FACS IF IHC (f) CF®555 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155625 FACS IF IHC (f) CF®543 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155633 FACS IF IHC (f) CF®568 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155623 FACS IF IHC (f) CF®640R Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155631 FACS IF IHC (f) CF®660R Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155639 FACS IF IHC (f) CF®680R Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155654 FACS IF IHC (f) Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155646 FACS IF IHC (f) Alkaline Phosphatase (AP) Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155645 FACS IF IHC (f) APC Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155648 FACS IF IHC (f) Biotin Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155652 FACS IF IHC (f) PerCP Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155653 FACS IF IHC (f) PE Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155656 FACS IF IHC (f) Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155650 FACS IF IHC (f) HRP Mouse IgG2a, kappa N-Term 1A4 0

General

Antigen Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
Epitope N-Term
(149), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(401), (261), (204), (85), (72), (67), (58), (42), (32), (30), (29), (29), (27), (26), (13), (7), (4), (3), (2), (1), (1), (1)
Host Mouse
(236), (156), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Smooth Muscle Actin antibody is conjugated to CF405M
(17), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(208), (162), (129), (117), (97), (95), (63), (21), (19), (17), (14), (6), (4), (3), (3), (2), (1), (1), (1)
Supplier

Product Details anti-Smooth Muscle Actin Antibody

Target Details Smooth Muscle Actin Application Details Handling Images
Purpose Mouse Monoclonal anti-Smooth-Muscle-Actin (1A4), CF405M Conjugate
Characteristics Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Clone 1A4
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details Smooth Muscle Actin

Product Details anti-Smooth Muscle Actin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Smooth-Muscle-Actin (ACTA2 Antibody Abstract)
Background ACTA2|Actin Alpha 2 Smooth Muscle Aorta|Actin Alpha Smooth Muscle|Actin Aortic Smooth Muscle|Actin Vascular Smooth Muscle|ACTSA|ACTVS|Alpha 2 Actin|Alpha Cardiac Actin|Alpha-actin-2|ASMA|Cell Growth-inhibiting Gene 46 Protein|Growth Inhibiting Gene 46
Molecular Weight 42 kDa
Gene ID 59, 500483
UniProt P62736
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development

Application Details

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Optimal dilution for a specific application should be determined by user
Comment

Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions For Research Use only

Handling

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light