anti-Cow (Bovine) Smooth Muscle Actin antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Recommended Smooth Muscle Actin Antibody

Antigen
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
  • ASMA
  • SMactin
  • a-sma
  • aat6
  • actsa
  • mymy5
  • sm-actin
  • ACTA2
  • actc1
  • ACT-4
  • AAT6
  • ACTSA
  • MYMY5
  • 0610041G09Rik
  • Actvs
  • SMalphaA
  • a-SMA
  • alphaSMA
  • wu:fb63d03
  • asma
  • actin, alpha 2, smooth muscle, aorta L homeolog
  • actin, alpha 2, smooth muscle, aorta
  • acta2.L
  • ACTA2
  • acta2
  • Acta2
Epitope
N-Term
155
5
4
4
3
3
2
1
1
1
1
1
1
1
1
Reactivity
Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
416
271
210
90
77
72
63
46
36
35
34
33
31
30
14
7
4
3
2
1
1
1
Host
Mouse
246
160
17
Clonality
Monoclonal
Conjugate
This Smooth Muscle Actin antibody is un-conjugated
18
7
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)
213
173
137
123
99
95
64
21
20
18
14
8
7
4
3
2
2
1
1
1
Options
Catalog No. ABIN6295487
$ 575.30
Plus shipping costs $45.00

General

Antigen Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
Epitope N-Term
(155), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(416), (271), (210), (90), (77), (72), (63), (46), (36), (35), (34), (33), (31), (30), (14), (7), (4), (3), (2), (1), (1), (1)
Host Mouse
(246), (160), (17)
Clonality
Monoclonal
Conjugate This Smooth Muscle Actin antibody is un-conjugated
(18), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)
(213), (173), (137), (123), (99), (95), (64), (21), (20), (18), (14), (8), (7), (4), (3), (2), (2), (1), (1), (1)
Supplier

Product Details anti-Smooth Muscle Actin Antibody

Target Details Smooth Muscle Actin Application Details Handling Images
Purpose Mouse anti-Human/Baboon/Monkey/Cow/Pig/Sheep/Goat/Cat/Dog/Rabbit/Mouse/Rat/Guinea pig/Chicken. Smooth Muscle Actin Antibody [Sodium Azide Free]
Specificity Cytoplasmic
Purification An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.
Immunogen An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.
Plasmids, Primers & others

Target Details Smooth Muscle Actin

Product Details anti-Smooth Muscle Actin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Actin, aortic smooth muscle (ACTA2 Antibody Abstract)
Background

Target Description: Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.

Gene Symbol: ACTA2///ACTSA///ACTVS///GIG46

Gene ID 59
UniProt P62736
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development

Application Details

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling Images back to top
Application Notes Western blot: 0.5-1 μg/mL
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only

Handling

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Images back to top
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.