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|Antigen||Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies|
|Reactivity||Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This Smooth Muscle Actin antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Product Details anti-Smooth Muscle Actin AntibodyTarget Details Smooth Muscle Actin Application Details Handling Images
|Purification||Protein G affinity chromatography|
|Immunogen||An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.|
|Plasmids, Primers & others|
Target Details Smooth Muscle ActinProduct Details anti-Smooth Muscle Actin Antibody Application Details Handling Images back to top
|Alternative Name||Smooth Muscle Actin (ACTA2 Antibody Abstract)|
|Background||Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.|
|Pathways||Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development|
Application DetailsProduct Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling Images back to top
Variations in protocols, secondaries and substrates may require the Smooth muscle actin antibody to be titered up or down for optimal performance.
1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Images back to top
|Buffer||0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the Smooth muscle actin antibody at 2-8°C (aliquot/freeze the azide-free format).|
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