anti-Goat Smooth Muscle Actin antibody for Simple Western

Recommended Smooth Muscle Actin Antibody

Antigen
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
  • ASMA
  • SMactin
  • a-sma
  • aat6
  • actsa
  • mymy5
  • sm-actin
  • ACTA2
  • actc1
  • ACT-4
  • AAT6
  • ACTSA
  • MYMY5
  • 0610041G09Rik
  • Actvs
  • SMalphaA
  • a-SMA
  • alphaSMA
  • wu:fb63d03
  • asma
  • actin, alpha 2, smooth muscle, aorta L homeolog
  • actin, alpha 2, smooth muscle, aorta
  • acta2.L
  • ACTA2
  • acta2
  • Acta2
Epitope
N-Term
150
5
4
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
402
262
205
86
73
68
59
43
33
31
30
30
28
27
13
7
4
3
2
1
1
1
Host
Mouse
237
156
16
Clonality
Monoclonal
Conjugate
This Smooth Muscle Actin antibody is un-conjugated
17
7
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
208
162
130
118
97
95
64
21
19
17
14
6
4
3
3
2
1
1
1
Options
Catalog No. ABIN4280067
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General

Antigen Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
Epitope N-Term
(150), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(402), (262), (205), (86), (73), (68), (59), (43), (33), (31), (30), (30), (28), (27), (13), (7), (4), (3), (2), (1), (1), (1)
Host Mouse
(237), (156), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Smooth Muscle Actin antibody is un-conjugated
(17), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(208), (162), (130), (118), (97), (95), (64), (21), (19), (17), (14), (6), (4), (3), (3), (2), (1), (1), (1)
Supplier

Product Details anti-Smooth Muscle Actin Antibody

Target Details Smooth Muscle Actin Application Details Handling Images
Specificity Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Purification Protein A purified
Immunogen N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH
Clone 1A4-asm-1
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details Smooth Muscle Actin

Product Details anti-Smooth Muscle Actin Antibody Application Details Handling Images back to top
Antigen
Alternative Name alpha-Smooth Muscle Actin (ACTA2 Antibody Abstract)
Background Gene Symbol: ACTA2
Molecular Weight Theoretical MW: 42 kDa
Gene ID 59
UniProt P62736
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development

Application Details

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling Images back to top
Application Notes Western Blot 0.5-1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 μg/mL, Immunoprecipitation 0.5-1 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mLELISA (For coating, order NBP2-34522 (BSA free version of NBP2-33006))
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.