anti-Guinea Pig Smooth Muscle Actin antibody for Immunofluorescence

Recommended Smooth Muscle Actin Antibody

Antigen
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
  • ASMA
  • SMactin
  • a-sma
  • aat6
  • actsa
  • mymy5
  • sm-actin
  • ACTA2
  • actc1
  • ACT-4
  • AAT6
  • ACTSA
  • MYMY5
  • 0610041G09Rik
  • Actvs
  • SMalphaA
  • a-SMA
  • alphaSMA
  • wu:fb63d03
  • asma
  • actin, alpha 2, smooth muscle, aorta L homeolog
  • actin, alpha 2, smooth muscle, aorta
  • acta2.L
  • ACTA2
  • acta2
  • Acta2
Epitope
N-Term
149
5
4
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
401
261
204
85
72
67
58
42
32
30
29
29
27
26
13
7
4
3
2
1
1
1
Host
Mouse
236
156
16
Clonality
Monoclonal
Conjugate
This Smooth Muscle Actin antibody is un-conjugated
17
7
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
207
162
129
117
97
94
64
21
19
17
14
6
4
3
3
2
1
1
1
Options

Available images

Catalog No. ABIN3024126
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN3024124 FACS IF IHC (p) WB Mouse IgG2a, kappa SPM332 0
4 ABIN3024128 FACS IF IHC (p) WB Mouse IgG2a, kappa N-Term 1A4 0
4 ABIN3024125 FACS IF IHC (p) WB Mouse IgG2a, kappa SPM332 0
1 ABIN6155621 FACS IF IHC (f) CF405M Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155643 FACS IF IHC (f) CF®594 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155627 FACS IF IHC (f) CF®647 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155637 FACS IF IHC (f) CF®680 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155635 FACS IF IHC (f) CF®770 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155619 FACS IF IHC (f) CF®405S Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155641 FACS IF IHC (f) CF®488A Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155629 FACS IF IHC (f) CF®555 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155625 FACS IF IHC (f) CF®543 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155633 FACS IF IHC (f) CF®568 Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155623 FACS IF IHC (f) CF®640R Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155631 FACS IF IHC (f) CF®660R Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155639 FACS IF IHC (f) CF®680R Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155654 FACS IF IHC (f) Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155646 FACS IF IHC (f) Alkaline Phosphatase (AP) Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155645 FACS IF IHC (f) APC Mouse IgG2a, kappa N-Term 1A4 0
1 ABIN6155648 FACS IF IHC (f) Biotin Mouse IgG2a, kappa N-Term 1A4 0

General

Antigen Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
Epitope N-Term
(149), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(401), (261), (204), (85), (72), (67), (58), (42), (32), (30), (29), (29), (27), (26), (13), (7), (4), (3), (2), (1), (1), (1)
Host Mouse
(236), (156), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Smooth Muscle Actin antibody is un-conjugated
(17), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(207), (162), (129), (117), (97), (94), (64), (21), (19), (17), (14), (6), (4), (3), (3), (2), (1), (1), (1)
Supplier

Product Details anti-Smooth Muscle Actin Antibody

Target Details Smooth Muscle Actin Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.
Clone 1A4
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details Smooth Muscle Actin

Product Details anti-Smooth Muscle Actin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Smooth Muscle Actin (ACTA2 Antibody Abstract)
Background Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.
Gene ID 59
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development

Application Details

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling Images back to top
Application Notes Variations in protocols, secondaries and substrates may require the Smooth muscle actin antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the Smooth muscle actin antibody at 2-8°C (aliquot/freeze the azide-free format).

Images

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody (ABIN3024126) IHC: human leiomyosarcoma stained with Smooth muscle actin antibody (1A4).