anti-Guinea Pig Smooth Muscle Actin antibody for Western Blotting

Recommended Smooth Muscle Actin Antibody

Antigen
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
  • ASMA
  • SMactin
  • a-sma
  • aat6
  • actsa
  • mymy5
  • sm-actin
  • ACTA2
  • actc1
  • ACT-4
  • AAT6
  • ACTSA
  • MYMY5
  • 0610041G09Rik
  • Actvs
  • SMalphaA
  • a-SMA
  • alphaSMA
  • wu:fb63d03
  • asma
  • actin, alpha 2, smooth muscle, aorta L homeolog
  • actin, alpha 2, smooth muscle, aorta
  • acta2.L
  • ACTA2
  • acta2
  • Acta2
Epitope
AA 167-377
150
5
4
4
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Guinea Pig, Rabbit, Rhesus Monkey, Cow (Bovine), Horse (Equine), Chicken
401
261
204
85
72
67
59
43
33
30
30
30
28
27
12
7
4
3
2
1
1
Host
Rabbit
237
155
16
Clonality
Polyclonal
Conjugate
This Smooth Muscle Actin antibody is un-conjugated
17
7
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
207
161
130
118
97
95
64
21
19
16
14
6
4
3
3
2
1
1
1
Options

Available images

Catalog No. ABIN1857877
$ 342.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN3024126 FACS IF IHC (p) WB Mouse IgG2a, kappa N-Term 1A4 0
4 ABIN3024124 FACS IF IHC (p) WB Mouse IgG2a, kappa SPM332 0
4 ABIN3024128 FACS IF IHC (p) WB Mouse IgG2a, kappa N-Term 1A4 0
4 ABIN3024125 FACS IF IHC (p) WB Mouse IgG2a, kappa SPM332 0
1 ABIN6251798 FACS IF IHC WB Mouse IgG2a, kappa SPM332 0
1 ABIN6295487 FACS IF IHC (fp) WB Mouse N-Term 0
1 ABIN6295926 FACS IF IHC WB Mouse 0
-10.051895 ABIN4280083 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.051895 ABIN4280064 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a kappa N-Term SPM332 0
-13.051895 ABIN4280067 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.051895 ABIN4280100 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a kappa N-Term SPM332 0

General

Antigen Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
Epitope AA 167-377
(150), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Guinea Pig, Rabbit, Rhesus Monkey, Cow (Bovine), Horse (Equine), Chicken
(401), (261), (204), (85), (72), (67), (59), (43), (33), (30), (30), (30), (28), (27), (12), (7), (4), (3), (2), (1), (1)
Host Rabbit
(237), (155), (16)
Clonality
Polyclonal
Conjugate This Smooth Muscle Actin antibody is un-conjugated
(17), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(207), (161), (130), (118), (97), (95), (64), (21), (19), (16), (14), (6), (4), (3), (3), (2), (1), (1), (1)
Supplier

Product Details anti-Smooth Muscle Actin Antibody

Target Details Smooth Muscle Actin Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen ACTa2 (Ile167-Phe377)
Isotype IgG
Plasmids, Primers & others

Target Details Smooth Muscle Actin

Product Details anti-Smooth Muscle Actin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Actin Alpha 2, Smooth Muscle (ACTa2) (ACTA2 Antibody Abstract)
Background Alternative Names: ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development

Application Details

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling Images back to top
Application Notes
  • Western blotting: 0.5-2 μg/mL
  • Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
  • Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
  • Immunohistochemistry in paraffin section: 5-20 μg/mL
  • Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
  • Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody (ABIN1857877) anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody
Western Blotting (WB) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody (ABIN1857877) Figure. Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Heart Tissue; L...
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody (ABIN1857877) Figure. DAB staining on IHC-P; Samples:Human Stomach Cancer Tissue.
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody (ABIN1857877) Figure. DAB staining on IHC-P; Samples:Human Esophagus Tissue.
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody (ABIN1857877) Figure. DAB staining on IHC-P; Samples:Human Rectum Cancer Tissue.
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibody (ABIN1857877) Figure. DAB staining on IHC-P; Samples:Human Stomach Tissue.