anti-Mouse (Murine) Smooth Muscle Actin antibody for Cytometry by Time of Flight

Recommended Smooth Muscle Actin Antibody

Antigen
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
  • ASMA
  • SMactin
  • a-sma
  • aat6
  • actsa
  • mymy5
  • sm-actin
  • ACTA2
  • actc1
  • ACT-4
  • AAT6
  • ACTSA
  • MYMY5
  • 0610041G09Rik
  • Actvs
  • SMalphaA
  • a-SMA
  • alphaSMA
  • wu:fb63d03
  • asma
  • actin, alpha 2, smooth muscle, aorta L homeolog
  • actin, alpha 2, smooth muscle, aorta
  • acta2.L
  • ACTA2
  • acta2
  • Acta2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
402
262
205
86
73
68
59
43
33
31
30
30
28
27
13
7
4
3
2
1
1
1
Host
Mouse
237
156
16
Clonality
Monoclonal
Conjugate
This Smooth Muscle Actin antibody is un-conjugated
17
7
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
208
162
130
118
97
95
64
21
19
17
14
6
4
3
3
2
1
1
1
Options

Available images

Catalog No. ABIN4900586
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General

Antigen Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(402), (262), (205), (86), (73), (68), (59), (43), (33), (31), (30), (30), (28), (27), (13), (7), (4), (3), (2), (1), (1), (1)
Host Mouse
(237), (156), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Smooth Muscle Actin antibody is un-conjugated
(17), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(208), (162), (130), (118), (97), (95), (64), (21), (19), (17), (14), (6), (4), (3), (3), (2), (1), (1), (1)
Pubmed 47 references available
Supplier

Product Details anti-Smooth Muscle Actin Antibody

Target Details Smooth Muscle Actin Application Details Handling References for anti-Smooth Muscle Actin antibody (ABIN4900586) Images
Specificity Detects human alpha-Smooth Muscle Actin.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha-smooth muscle actin (1)
Clone 1A4
Isotype IgG2a
Plasmids, Primers & others

Target Details Smooth Muscle Actin

Product Details anti-Smooth Muscle Actin Antibody Application Details Handling References for anti-Smooth Muscle Actin antibody (ABIN4900586) Images back to top
Antigen
Alternative Name ACTA2 (ACTA2 Antibody Abstract)
Background Alternative Names: AAT6, ACTA2, Actin alpha 2, actin, alpha 2, smooth muscle, aorta, actin, aortic smooth muscle, ACTSA, ACTVS, alpha 2 actin, alpha SMA, alpha smooth muscle actin, alpha-actin-2, alpha-SMA, alphaSmooth Muscle Actin, a-sma, Cell growth-inhibiting gene 46 protein, growth-inhibiting gene 46, MYMY5, SMA, smooth muscle actin
Gene ID 59
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development

Application Details

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling References for anti-Smooth Muscle Actin antibody (ABIN4900586) Images back to top
Application Notes Western Blot 0.1-0.25 μg/mL
Simple Western 10 μg/mL
Immunohistochemistry 8-25 μg/mL
Intracellular Staining by Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details References for anti-Smooth Muscle Actin antibody (ABIN4900586) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Smooth Muscle Actin antibody (ABIN4900586)

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Handling Images back to top
Product cited in:

Kanda, Noda, Hirose, Ishida: "TGF-β-SNAIL axis induces Müller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane." in: Scientific reports, Vol. 9, Issue 1, pp. 673, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Masuda, Nakashima, Namba, Yamaguchi, Sakamoto, Horimasu, Miyamoto, Iwamoto, Fujitaka, Miyata, Hamada, Okada, Hattori: "Inhibition of PAI-1 limits chemotherapy resistance in lung cancer through suppressing myofibroblast characteristics of cancer-associated fibroblasts." in: Journal of cellular and molecular medicine, Vol. 23, Issue 4, pp. 2984-2994, 2019 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Guerrero-Juarez, Dedhia, Jin, Ruiz-Vega, Ma, Liu, Yamaga, Shestova, Gay, Yang, Kessenbrock, Nie, Pear, Cotsarelis, Plikus: "Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds." in: Nature communications, Vol. 10, Issue 1, pp. 650, 2019 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Bikkuzin, Shi, Sun, Guo, Jin, Han, Pavlov, Zhang: "Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells." in: Stem cell research, Vol. 37, pp. 101409, 2019 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Takahashi, Sakakura, Kudo, Toyoda, Kaira, Oyama, Chikamatsu: "Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages." in: Oncotarget, Vol. 8, Issue 5, pp. 8633-8647, 2018 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Human).

Wong, Lim, Hung, Jackson, Khan, Van Bergen, De Smit, Liang, Kearns, Clarke, Mackey, Hewitt, Trounce, Pébay: "Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy." in: Aging, Vol. 9, Issue 4, pp. 1341-1350, 2018 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Zhou, Huang, Xie, Mei: "Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-κB and MURF1 levels." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3674-3680, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Espitia, Chatelais, Steenman, Charrier, Maurel, Georges, Houlgatte, Verrecchia, Ory, Lamoureux, Heymann, Gouëffic, Quillard: "Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification." in: PLoS ONE, Vol. 13, Issue 1, pp. e0191976, 2018 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Barnes, Salas, Mokhtari, Dolstra, Pedrosa, Lachman: "Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells." in: Molecular autism, Vol. 9, pp. 44, 2018 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Li, Li, Li, Wei, He, Huang, Wang, Xia, Xu, Li, Wang, Yang, Zhang, Xu: "Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition." in: Cell death & disease, Vol. 9, Issue 9, pp. 854, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kaur, Abu-Shahba, Paananen, Hongisto, Hiidenmaa, Skottman, Seppänen-Kaijansinkko, Mannerström: "Small non-coding RNA landscape of extracellular vesicles from human stem cells." in: Scientific reports, Vol. 8, Issue 1, pp. 15503, 2018

Reisler, Zeng, Schellhase, Bearss, Warren, Trefry, Christopher, Kortepeter, Bavari, Cardile: "Ebola Virus Causes Intestinal Tract Architectural Disruption and Bacterial Invasion in Non-Human Primates." in: Viruses, Vol. 10, Issue 10, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kwack, Yang, Won, Kim, Kim, Sung: "Establishment and characterization of five immortalized human scalp dermal papilla cell lines." in: Biochemical and biophysical research communications, Vol. 496, Issue 2, pp. 346-351, 2018 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Reichman, Park, Man, Redmond, Chao, Harvey, Taketo, Rosenwaks, James: "Wnt inhibition promotes vascular specification of embryonic cardiac progenitors." in: Development (Cambridge, England), Vol. 145, Issue 1, 2018 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Smith, Hollidge, Daye, Zeng, Blancett, Kuszpit, Bocan, Koehler, Coyne, Minogue, Kenny, Chi, Yim, Miller, Schmaljohn, Bavari, Golden: "Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon." in: PLoS neglected tropical diseases, Vol. 11, Issue 1, pp. e0005296, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Hrstka, Li, Nelson: "NOTCH1-Dependent Nitric Oxide Signaling Deficiency in Hypoplastic Left Heart Syndrome Revealed Through Patient-Specific Phenotypes Detected in Bioengineered Cardiogenesis." in: Stem cells (Dayton, Ohio), Vol. 35, Issue 4, pp. 1106-1119, 2017 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Dong, Kanda, Noda, Saito, Ishida: "Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane." in: Scientific reports, Vol. 7, pp. 44266, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Kamata, Tsukasaki, Sakai, Ikebe, Wang, Jeffers, Boren, Owens, Suzuki, Higashihara, Idell, Tucker, Ikebe: "KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis." in: Scientific reports, Vol. 7, Issue 1, pp. 4556, 2017 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Lombardi, Mantione, Baccellieri, Ferrara, Castellano, Chiesa, Alfieri, Foglieni: "P2X7 receptor antagonism modulates IL-1β and MMP9 in human atherosclerotic vessels." in: Scientific reports, Vol. 7, Issue 1, pp. 4872, 2017 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Human).

Wong, Hung, Jackson, Singh, Khan, Liang, Kearns, Nguyen, Conquest, Daniszewski, Hewitt, Pébay: "Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors." in: Stem cell research, Vol. 22, pp. 13-15, 2017 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Images

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Handling References for anti-Smooth Muscle Actin antibody (ABIN4900586) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (ABIN4900586) Detection of alpha-Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Hu...
Immunocytochemistry (ICC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (ABIN4900586) alpha-Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. alpha-Smooth Muscle Ac...
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (ABIN4900586) alpha-Smooth Muscle Actin in Human Breast Cancer Tissue. alpha-Smooth Muscle Actin wa...
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (ABIN4900586) alpha-Smooth Muscle Actin in Human Heart. alpha-Smooth Muscle Actin was detected in i...
Simple Western (SimWes) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (ABIN4900586) Detection of Human alpha-Smooth Muscle Actin by Simple WesternTM. Simple Western lane...
Western Blotting (WB) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (ABIN4900586) Detection of Human alpha-Smooth Muscle Actin by Western Blot. Western blot shows lysa...
Western Blotting (WB) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (ABIN4900586) Detection of Mouse and Rat alpha-Smooth Muscle Actin by Western Blot. Western blot sh...