anti-Sheep (Ovine) Smooth Muscle Actin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Smooth Muscle Actin Antibody

Antigen
Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
  • ASMA
  • SMactin
  • a-sma
  • aat6
  • actsa
  • mymy5
  • sm-actin
  • ACTA2
  • actc1
  • ACT-4
  • AAT6
  • ACTSA
  • MYMY5
  • 0610041G09Rik
  • Actvs
  • SMalphaA
  • a-SMA
  • alphaSMA
  • wu:fb63d03
  • asma
  • actin, alpha 2, smooth muscle, aorta L homeolog
  • actin, alpha 2, smooth muscle, aorta
  • acta2.L
  • ACTA2
  • acta2
  • Acta2
Epitope
N-Term
155
5
4
4
3
3
2
1
1
1
1
1
1
1
1
Reactivity
Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
416
271
210
90
77
72
63
46
36
35
34
33
31
30
14
7
4
3
2
1
1
1
Host
Mouse
246
160
17
Clonality
Monoclonal
Conjugate
This Smooth Muscle Actin antibody is un-conjugated
18
7
6
6
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
213
173
137
123
99
94
64
21
20
18
14
8
7
4
3
3
2
1
1
1
Options

Available images

Catalog No. ABIN3024126
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN3024127 IHC (p) Mouse IgG2a, kappa N-Term 1A4 0
4 ABIN3024128 FACS IF IHC (p) WB Mouse IgG2a, kappa N-Term 1A4 0
-0.7914214 ABIN269603 IHC (p) IHC Rabbit IgG N-Term Polyclonal 2
-7.104517 ABIN5945109 FACS ICC IF IHC IHC (p) Janelia Fluor® 549 Mouse IgG2a kappa N-Term 1A4-asm-1 0
-8.604517 ABIN4280073 ELISA FACS ICC IF IHC (p) Alexa Fluor 488 Mouse IgG2a kappa N-Term 1A4-asm-1 1
-8.604517 ABIN4280072 ELISA FACS ICC IF IHC (p) Alexa Fluor 405 Mouse IgG2a kappa N-Term 1A4-asm-1 1
-10.104517 ABIN4280075 ELISA FACS ICC IF IHC (p) Alexa Fluor 700 Mouse IgG2a kappa N-Term 1A4-asm-1 0
-10.104517 ABIN4280083 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a kappa N-Term 1A4-asm-1 0
-10.104517 ABIN4280091 ELISA FACS ICC IF IHC (p) Alexa Fluor 647 Mouse IgG2a kappa N-Term SPM332 0
-13.104517 ABIN4280064 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a kappa N-Term SPM332 0
-13.104517 ABIN4280067 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.104517 ABIN4280074 ELISA FACS ICC IF IHC (p) Alexa Fluor 647 Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.104517 ABIN4280078 ELISA FACS ICC IF IHC (p) DyLight 650 Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.104517 ABIN4280079 ELISA FACS ICC IF IHC (p) DyLight 680 Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.104517 ABIN4280080 ELISA FACS ICC IF IHC (p) DyLight 488 Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.104517 ABIN4280082 ELISA FACS ICC IF IHC (p) DyLight 755 Mouse IgG2a kappa N-Term 1A4-asm-1 0
-13.104517 ABIN4280089 ELISA FACS ICC IF IHC (p) Alexa Fluor 405 Mouse IgG2a kappa N-Term SPM332 0
-13.104517 ABIN4280094 ELISA ICC IF IHC (p) Biotin Mouse IgG2a kappa N-Term SPM332 0
-13.104517 ABIN4280095 ELISA FACS ICC IF IHC (p) DyLight 650 Mouse IgG2a kappa N-Term SPM332 0
-13.104517 ABIN4280096 ELISA FACS ICC IF IHC (p) DyLight 680 Mouse IgG2a kappa N-Term SPM332 0

General

Antigen Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antibodies
Epitope N-Term
(155), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Baboon, Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(416), (271), (210), (90), (77), (72), (63), (46), (36), (35), (34), (33), (31), (30), (14), (7), (4), (3), (2), (1), (1), (1)
Host Mouse
(246), (160), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Smooth Muscle Actin antibody is un-conjugated
(18), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(213), (173), (137), (123), (99), (94), (64), (21), (20), (18), (14), (8), (7), (4), (3), (3), (2), (1), (1), (1)
Supplier

Product Details anti-Smooth Muscle Actin Antibody

Target Details Smooth Muscle Actin Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.
Clone 1A4
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details Smooth Muscle Actin

Product Details anti-Smooth Muscle Actin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Smooth Muscle Actin (ACTA2 Antibody Abstract)
Background Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.
Gene ID 59
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development

Application Details

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Handling Images back to top
Application Notes Variations in protocols, secondaries and substrates may require the Smooth muscle actin antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the Smooth muscle actin antibody at 2-8°C (aliquot/freeze the azide-free format).

Images

Product Details anti-Smooth Muscle Actin Antibody Target Details Smooth Muscle Actin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody (ABIN3024126) IHC: human leiomyosarcoma stained with Smooth muscle actin antibody (1A4).