anti-Human ABCC8 antibody for Immunofluorescence

Recommended ABCC8 Antibody (supplied by: Log in to see )

Antigen
ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) Antibodies
  • ABC36
  • HHF1
  • HI
  • HRINS
  • MRP8
  • PHHI
  • SUR
  • SUR1
  • SUR1delta2
  • TNDM2
  • D930031B21Rik
  • Sur
  • Sur1
  • ATP binding cassette subfamily C member 8
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 8
  • ABCC8
  • Abcc8
Epitope
AA 1548-1582, C-Term
71
66
14
4
4
3
3
2
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
107
98
85
70
4
3
2
2
2
1
1
1
1
Host
Mouse
95
29
17
Clonality
Monoclonal
Conjugate
This ABCC8 antibody is un-conjugated
9
9
7
6
5
4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
128
93
64
44
36
21
9
3
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Pubmed
2 references available

Available images

Immunofluorescence (fixed cells) (IF/ICC) image for anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) (AA 1548-1582), (C-Term) antibody (ABIN1027723)
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) (AA 1548-1582), (C-Term) antibody (ABIN1027723)
Catalog No. ABIN1027723
$ 453.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.754766 ABIN2482993 ICC IF IHC WB Alkaline Phosphatase (AP) Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482996 ICC IF IHC WB FITC Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482997 ICC IF IHC WB HRP Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482989 ICC IF IHC WB Atto 633 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482992 ICC IF IHC WB Atto 700 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482987 ICC IF IHC WB Atto 565 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482986 ICC IF IHC WB Atto 488 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482985 ICC IF IHC WB Atto 390 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482988 ICC IF IHC WB Atto 594 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482990 ICC IF IHC WB Atto 655 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482991 ICC IF IHC WB Atto 680 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482994 ICC IF IHC WB APC Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482995 ICC IF IHC WB Biotin Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482999 ICC IF IHC WB PerCP Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2482998 ICC IF IHC WB PE-Atto 594 Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2483000 ICC IF IHC WB PE Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0
18.754766 ABIN2483001 ICC IF IHC WB Streptavidin Mouse IgG1 AA 1548-1582, C-Term Log in to see S289-16 0

General

Antigen ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) Antibodies
Epitope AA 1548-1582, C-Term
(71), (66), (14), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human, Mouse (Murine), Rat (Rattus)
(107), (98), (85), (70), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(95), (29), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ABCC8 antibody is un-conjugated
(9), (9), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(128), (93), (64), (44), (36), (21), (9), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-ABCC8 Antibody

Target Details ABCC8 Application Details Handling References for anti-ABCC8 antibody (ABIN1027723) Images
Specificity Detects ~160 kDa. Does not cross-react with SUR2B.
Purification Protein G Purified
Immunogen Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1
Clone S289-16
Isotype IgG1
Plasmids, Primers & others

Target Details ABCC8

Product Details anti-ABCC8 Antibody Application Details Handling References for anti-ABCC8 antibody (ABIN1027723) Images back to top
Antigen
Alternative Name SUR1 (ABCC8 Antibody Abstract)
Background Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).
Gene ID 25559
NCBI Accession NP_037171
UniProt Q09429
Pathways Negative Regulation of Hormone Secretion

Application Details

Product Details anti-ABCC8 Antibody Target Details ABCC8 Handling References for anti-ABCC8 antibody (ABIN1027723) Images back to top
Application Notes
  • WB (1:1000)
  • IHC (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SMC-409 was sufficient for detection of SUR1 in 20 μg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Restrictions For Research Use only

Handling

Product Details anti-ABCC8 Antibody Target Details ABCC8 Application Details References for anti-ABCC8 antibody (ABIN1027723) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-ABCC8 antibody (ABIN1027723)

Product Details anti-ABCC8 Antibody Target Details ABCC8 Application Details Handling Images back to top
Background publications

Nichols: "KATP channels as molecular sensors of cellular metabolism." in: Nature, Vol. 440, Issue 7083, pp. 470-6, 2006

Campbell, Sansom, Ashcroft: "Potassium channel regulation." in: EMBO reports, Vol. 4, Issue 11, pp. 1038-42, 2003

Images

Product Details anti-ABCC8 Antibody Target Details ABCC8 Application Details Handling References for anti-ABCC8 antibody (ABIN1027723) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) (AA 1548-1582), (C-Term) antibody (ABIN1027723) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Anti...
Western Blotting (WB) image for anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8) (AA 1548-1582), (C-Term) antibody (ABIN1027723) Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protei...