anti-Human Chromogranin A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivity
Human, Rat (Rattus)
999
315
258
206
167
123
12
11
6
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
684
316
22
7
1
1
Clonality
Monoclonal
Conjugate
Un-conjugated
40
35
31
31
27
16
16
16
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
14
12
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
680
630
458
403
303
212
211
196
168
102
16
13
13
8
7
3
2
1
1
Supplier
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Catalog No. ABIN3024534
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.117413 ABIN364359 ELISA IHC IHC (p) WB Mouse IgG1, kappa AA 19-131 Log in to see 3A5 0
13 ABIN1497513 IHC IHC (p) WB Mouse IgG1 Log in to see 1H2 0
13 ABIN1497514 IHC IHC (p) WB Mouse IgG1 Log in to see 1B7 0
13 ABIN1497515 IHC IHC (p) WB Mouse IgG1 Log in to see 2D6 0
13 ABIN3024531 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see CHGA-777 0
13 ABIN3024537 FACS IF IHC (p) Mouse IgG2b, kappa Log in to see CGA-413 0
13 ABIN3024525 FACS IF IHC (p) Mouse IgG Log in to see CGA-413-CHGA-777-CHGA-798 0
12.755764 ABIN285904 ICC IHC (fro) IHC (p) Rabbit IgG1 Log in to see Polyclonal 0
12.755764 ABIN283997 IHC (fro) IF IHC (p) Mouse IgG1 Log in to see PHE5 0
12.755764 ABIN283994 IHC (fro) IHC (p) Rabbit IgG Log in to see SP12 0
12.755764 ABIN283998 IHC (fro) IHC (p) Mouse IgG1 Log in to see LK2H10 0
12.755764 ABIN5621868 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
12.755764 ABIN283995 IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see LK2H10 0
12.755764 ABIN283996 IHC (fro) IF IHC (p) Mouse IgG1 Log in to see PHE5 0
11.117413 ABIN214090 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
10 ABIN574256 ELISA IHC IHC (p) WB Goat AA 85-97 Log in to see Polyclonal 0
10 ABIN782319 EIA IHC (p) WB Mouse IgG1 AA 19-131 Log in to see 3A5 0
10 ABIN1497512 IF IHC IHC (p) WB Mouse IgG1 Log in to see 2F2 0
10 ABIN1497509 IF IHC IHC (p) WB Mouse IgG1 Log in to see 1G3 0
10 ABIN1497510 IF IHC IHC (p) WB Mouse IgG1 Log in to see 1H9 0

General

Antigen Chromogranin A (CHGA) Antibodies
Reactivity Human, Rat (Rattus)
(999), (315), (258), (206), (167), (123), (12), (11), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(684), (316), (22), (7), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(40), (35), (31), (31), (27), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(680), (630), (458), (403), (303), (212), (211), (196), (168), (102), (16), (13), (13), (8), (7), (3), (2), (1), (1)
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Product details

Target Details Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the Chromogranin A antibody.
Clone CHGA-798
Isotype IgG1, kappa

Target Details

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Antigen
Alternative Name Chromogranin A (CHGA Antibody Abstract)
Background Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Optimal dilution of the Chromogranin A antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

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Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-fixed, paraffin-embedded human adrenal gland stained with Chromogranin A ant...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-fixed, paraffin-embedded human parathyroid stained with Chromogranin A antib...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-paraffin human pancreas stained with Chromogranin A antibody (CHGA/798)
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-paraffin human SC lung carcinoma stained with Chromogranin A antibody (CHGA/...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024534) Formalin-paraffin rat pancreas stained with Chromogranin A antibody (CHGA/798)