anti-Pig (Porcine) Chromogranin A antibody for Flow Cytometry

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivity
Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
999
315
257
205
166
123
12
11
6
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
684
316
22
7
1
1
Clonality
Monoclonal
Conjugate
Un-conjugated
40
35
31
31
27
16
16
16
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
14
12
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
680
630
458
403
303
212
211
196
168
102
16
13
13
8
7
3
2
1
1
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Catalog No. ABIN3024525
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6160775 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see LK2H10 0
7 ABIN6160782 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see LK2H10 0
7 ABIN6160783 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see LK2H10 0
7 ABIN6160786 FACS IF IHC (f) Mouse IgG1, kappa Log in to see LK2H10 0
7 ABIN3024549 FACS IF IHC (p) Mouse IgG1, kappa Log in to see LK2H10 0
4 ABIN3024547 FACS IF IHC (p) Mouse IgG1, kappa Log in to see SPM339 0
1 ABIN6160675 FACS IF IHC (f) CF405M Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160679 FACS IF IHC (f) CF®543 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160687 FACS IF IHC (f) CF®568 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160689 FACS IF IHC (f) CF®770 Mouse IgG Log in to see LK2H10 PHE5 CGA414 0
1 ABIN6160713 FACS IF IHC (f) CF405M Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160723 FACS IF IHC (f) CF®660R Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160725 FACS IF IHC (f) CF®568 Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160735 FACS IF IHC (f) CF®594 Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160733 FACS IF IHC (f) CF®488A Mouse IgG1 Log in to see LK2H10 PHE5 0
1 ABIN6160757 FACS IF IHC (f) CF®647 Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160755 FACS IF IHC (f) CF®543 Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160761 FACS IF IHC (f) CF®660R Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160765 FACS IF IHC (f) CF®770 Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160773 FACS IF IHC (f) CF®594 Mouse IgG1, kappa Log in to see LK2H10 0

General

Antigen Chromogranin A (CHGA) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(999), (315), (257), (205), (166), (123), (12), (11), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(684), (316), (22), (7), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(40), (35), (31), (31), (27), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(680), (630), (458), (403), (303), (212), (211), (196), (168), (102), (16), (13), (13), (8), (7), (3), (2), (1), (1)
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Product details

Target Details Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the Chromogranin A antibody cocktail.
Clone CGA-413-CHGA-777-CHGA-798
Isotype IgG

Target Details

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Antigen
Alternative Name Chromogranin A (CHGA Antibody Abstract)
Background Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Optimal dilution of the Chromogranin A antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

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Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Chromogranin A antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded adrenal gland stained with Chromogranin A antibody ...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded pancreas stained with Chromogranin A antibody cocktail
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded parathyroid stained with Chromogranin A antibody (C...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded pheochromocytoma stained with Chromogranin A antibo...