anti-Pig (Porcine) Chromogranin A antibody for Flow Cytometry

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivity
Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
717
204
187
130
121
57
15
12
7
6
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
477
238
22
7
1
1
Clonality
Monoclonal
Conjugate
Un-conjugated
27
22
18
18
17
17
16
16
16
16
16
16
16
16
16
15
13
8
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
511
399
385
327
219
216
171
117
110
31
13
13
7
4
3
2
2
1
1
1
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Catalog No. ABIN3024525
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3024549 FACS IF IHC (p) Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN4298451 FACS APC Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298417 FACS APC Mouse IgG1 kappa Log in to see LK2H10 0
1 ABIN4298468 FACS APC Mouse IgG1 kappa Log in to see SPM339 0
1 ABIN3024547 FACS IF IHC (p) Mouse IgG1, kappa Log in to see SPM339 0
1 ABIN2739940 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN4298364 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see LK2H10 0
1 ABIN4298365 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see LK2H10 0
1 ABIN4298367 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM339 0
1 ABIN4298366 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see LK2H10 0
1 ABIN4298369 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM339 0
1 ABIN4298368 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM339 0
1 ABIN4298396 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298397 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298398 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298448 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298449 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298450 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298453 FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0
1 ABIN4298455 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG Log in to see LK2H10-PHE5-CGA-414 0

General

Antigen Chromogranin A (CHGA) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(717), (204), (187), (130), (121), (57), (15), (12), (7), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(477), (238), (22), (7), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(27), (22), (18), (18), (17), (17), (16), (16), (16), (16), (16), (16), (16), (16), (16), (15), (13), (8), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(511), (399), (385), (327), (219), (216), (171), (117), (110), (31), (13), (13), (7), (4), (3), (2), (2), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the Chromogranin A antibody cocktail.
Clone CGA-413-CHGA-777-CHGA-798
Isotype IgG

Target Details

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Antigen
Alternative Name Chromogranin A (CHGA Antibody Abstract)
Background Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Optimal dilution of the Chromogranin A antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

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Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Chromogranin A antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded adrenal gland stained with Chromogranin A antibody ...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded pancreas stained with Chromogranin A antibody cocktail
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded parathyroid stained with Chromogranin A antibody (C...
Immunohistochemistry (IHC) image for anti-Chromogranin A (CHGA) antibody (ABIN3024525) Formalin-fixed, paraffin-embedded pheochromocytoma stained with Chromogranin A antibo...