anti-Human HADH antibody for Immunofluorescence

Recommended HADH Antibody (supplied by: Log in to see )

Antigen
Hydroxyacyl-CoA Dehydrogenase (HADH) Antibodies
  • HAD
  • HADHSC
  • hadhsc
  • wu:fb66a11
  • zgc:86777
  • Hadhsc
  • HADH1
  • HCDH
  • HHF4
  • MSCHAD
  • SCHAD
  • AA409008
  • AU019341
  • AW742602
  • Schad
  • hydroxyacyl-CoA dehydrogenase
  • hydroxyacyl-CoA dehydrogenase L homeolog
  • hydroxyacyl-Coenzyme A dehydrogenase
  • 3-hydroxyacyl-CoA dehydrogenase (short-chain)
  • Short chain 3-hydroxyacyl-CoA dehydrogenase
  • short chain 3-hydroxyacyl-coa dehydrogenase
  • putative short chain 3-hydroxyacyl-CoA dehydrogenase
  • HADH
  • hadh
  • hadh.L
  • Hadh
  • CNI02410
  • Tc00.1047053509717.90
  • Tc00.1047053510105.240
  • LMJF_36_1140
Epitope
AA 205-315
20
15
9
6
5
5
4
3
3
2
2
2
2
1
1
Reactivity
Human
110
66
58
10
8
5
4
4
4
3
3
3
1
Host
Mouse
80
36
18
1
Clonality
Monoclonal
Conjugate
This HADH antibody is un-conjugated
5
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
109
49
36
27
26
21
20
9
6
4
3
3
3
2
Options
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Catalog No. ABIN396238
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.106688 ABIN2856051 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
12.106688 ABIN561223 IF IHC (p) IP ELISA WB Mouse IgG2b kappa AA 205-314, partial Log in to see 4B5 3
12.106688 ABIN782407 EIA IF IHC (p) IP WB Mouse IgG2b AA 205-315 Log in to see 4B5 0
12.106688 ABIN516344 IF IP WB Rabbit AA 1-314, full length Log in to see Polyclonal 0
12.106688 ABIN516345 IF WB Rabbit AA 1-314, full length Log in to see Polyclonal 0
12.106688 ABIN4316539 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
12.106688 ABIN516343 IF WB Mouse AA 1-314, full length Log in to see Polyclonal 0
12.106688 ABIN4316538 ICC IF IHC IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 0
12.106688 ABIN4316537 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
12.106688 ABIN5077083 ICC IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN790535 ELISA IF IHC IHC (p) IP WB Mouse IgG2b, kappa AA 205-315 Log in to see 4B5 0
1 ABIN2471419 IF IP IHC ELISA WB Mouse IgG2b kappa AA 205-315 Log in to see 4B5 0
1 ABIN1723878 IF IHC (p) IP ELISA WB Mouse IgG2b kappa AA 205-315 Log in to see 4B5 0
1 ABIN4903863 IF IHC WB Mouse IgG Log in to see 0
1 ABIN5951265 ICC IF IHC WB Mouse IgG1 C-Term Log in to see D10-E7 0
1 ABIN2278758 IF IP IHC ELISA WB Mouse IgG2b kappa AA 205-315 Log in to see 4B5 0
1 ABIN2278806 IF IP IHC ELISA WB Mouse IgG2b kappa AA 205-314 Log in to see 4B5 0

General

Antigen Hydroxyacyl-CoA Dehydrogenase (HADH) Antibodies
Epitope AA 205-315
(20), (15), (9), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Reactivity Human
(110), (66), (58), (10), (8), (5), (4), (4), (4), (3), (3), (3), (1)
Host Mouse
(80), (36), (18), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HADH antibody is un-conjugated
(5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(109), (49), (36), (27), (26), (21), (20), (9), (6), (4), (3), (3), (3), (2)
Pubmed 5 references available
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Product Details anti-HADH Antibody

Target Details HADH Application Details Handling References for anti-HADH antibody (ABIN396238) Images
Specificity HADHSC (NP_005318, 205 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: HADHSC (NP_005318, 205 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 4B5
Isotype IgG2b kappa
Plasmids, Primers & others

Target Details HADH

Product Details anti-HADH Antibody Application Details Handling References for anti-HADH antibody (ABIN396238) Images back to top
Antigen
Alternative Name HADHSC (HADH Antibody Abstract)
Background

Other Names: Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial, HCDH, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, Short-chain 3-hydroxyacyl-CoA dehydrogenase, HADH, HAD, HADHSC, SCHAD

Gene Description: hydroxyacyl-Coenzyme A dehydrogenase

Gene Summary: This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq

Molecular Weight 34278 DA
Gene ID 3033
NCBI Accession NP_005318, NP_001171634
UniProt Q16836
Pathways Negative Regulation of Hormone Secretion, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-HADH Antibody Target Details HADH Handling References for anti-HADH antibody (ABIN396238) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.

Restrictions For Research Use only

Handling

Product Details anti-HADH Antibody Target Details HADH Application Details References for anti-HADH antibody (ABIN396238) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-HADH antibody (ABIN396238)

Product Details anti-HADH Antibody Target Details HADH Application Details Handling Images back to top
Background publications

Kalsi, Kuo, Aliev, Alexander, McMichael, Patterson, Walsh, Zhao, Schuckit, Nurnberger, Edenberg, Kramer, Hesselbrock, Tischfield, Vladimirov, Prescott, Dick, Kendler, Riley: "A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts." in: Human molecular genetics, Vol. 19, Issue 12, pp. 2497-506, 2010

Di Candia, Gessi, Pepe, Sogno Valin, Mangano, Chiumello, Gianolli, Proverbio, Mora: "Identification of a diffuse form of hyperinsulinemic hypoglycemia by 18-fluoro-L-3,4 dihydroxyphenylalanine positron emission tomography/CT in a patient carrying a novel mutation of the HADH gene." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 160, Issue 6, pp. 1019-23, 2009

van Hove, Hansen, Dekker, Reiling, Nijpels, Jørgensen, Borch-Johnsen, Hamid, Heine, Pedersen, Maassen, t Hart: "The HADHSC gene encoding short-chain L-3-hydroxyacyl-CoA dehydrogenase (SCHAD) and type 2 diabetes susceptibility: the DAMAGE study." in: Diabetes, Vol. 55, Issue 11, pp. 3193-6, 2006

Kimura, Wakamatsu, Suzuki, Ota, Nishikawa, Yamashita, Yamamoto, Sekine, Tsuritani, Wakaguri, Ishii, Sugiyama, Saito, Isono, Irie, Kushida, Yoneyama, Otsuka, Kanda, Yokoi, Kondo, Wagatsuma, Murakawa et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006

Yang, He, Schulz: "3-Hydroxyacyl-CoA dehydrogenase and short chain 3-hydroxyacyl-CoA dehydrogenase in human health and disease." in: The FEBS journal, Vol. 272, Issue 19, pp. 4874-83, 2005

Images

Product Details anti-HADH Antibody Target Details HADH Application Details Handling References for anti-HADH antibody (ABIN396238) back to top
Supplier Images
Western Blotting (WB) image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell (antibody concentrat...
Immunohistochemistry (IHC) image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (Image 2)
Western Blotting (WB) image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) HADHSC monoclonal antibody (M01), clone 4B5 Western Blot analysis of HADHSC expressio...
Immunohistochemistry (IHC) image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded...
Immunofluorescence (IF) image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentra...
Immunoprecipitation (IP) image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody an...
ELISA image for anti-Hydroxyacyl-CoA Dehydrogenase (HADH) (AA 205-315) antibody (ABIN396238) Detection limit for recombinant GST tagged HADHSC is approximately 0.03ng/ml as a cap...