anti-Human REST antibody for ELISA

Recommended REST Antibody (supplied by: Log in to see )

Antigen
RE1-Silencing Transcription Factor (REST) Antibodies
  • NRSF
  • XBR
  • 2610008J04Rik
  • AA407358
  • REST
  • xbr
  • nrsf
  • rest
  • RE1 silencing transcription factor
  • RE1-silencing transcription factor
  • REST
  • Rest
  • rest
Epitope
Internal Region
8
4
2
2
1
1
1
1
1
1
1
Reactivity
Human
27
7
6
2
1
1
1
1
1
1
1
Host
Goat
23
4
1
Clonality
Polyclonal
Conjugate
This REST antibody is un-conjugated
2
2
1
1
1
1
Application
ELISA, Western Blotting (WB)
23
11
8
7
7
6
3
1
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Catalog No. ABIN5539667
$ 358.02
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5688222 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN1918615 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 542-570 Log in to see Polyclonal 0
1 ABIN1918616 ELISA WB APC Rabbit IgG AA 542-570 Log in to see Polyclonal 0
1 ABIN1918617 ELISA WB Biotin Rabbit IgG AA 542-570 Log in to see Polyclonal 0
1 ABIN1918619 ELISA WB PE Rabbit IgG AA 542-570 Log in to see Polyclonal 0
1 ABIN1918618 ELISA WB FITC Rabbit IgG AA 542-570 Log in to see Polyclonal 0
1 ABIN2751251 ELISA IP WB FITC Rabbit IgG AA 1000-1200 Log in to see Polyclonal 0
1 ABIN1918620 ELISA WB HRP Rabbit IgG AA 542-570 Log in to see Polyclonal 0
1 ABIN2751250 ELISA IP WB Rabbit IgG AA 1000-1200 Log in to see Polyclonal 0
1 ABIN5892126 ELISA Goat Log in to see Polyclonal 0
1 ABIN5558149 ELISA IP WB Biotin Rabbit Log in to see Polyclonal 0

General

Antigen RE1-Silencing Transcription Factor (REST) Antibodies
Epitope Internal Region
(8), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(27), (7), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Host Goat
(23), (4), (1)
Clonality
Polyclonal
Conjugate This REST antibody is un-conjugated
(2), (2), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(23), (11), (8), (7), (7), (6), (3), (1)
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Product Details anti-REST Antibody

Target Details REST Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2816361
Sequence PETPGAEDKG KSSK
Specificity Important Comments: Reported variants represent identical protein: NP_001180437.1, NP_005603.3

Western Blot Results: Approx 140 kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122 kDa according to NP_005603.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recomme

Cross-Reactivity (Details) Expected Species Cross Reactivity: Human
Predicted Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Plasmids, Primers & others

Target Details REST

Product Details anti-REST Antibody Application Details Handling Images back to top
Antigen
Alternative Name RE1-silencing transcription factor (REST Antibody Abstract)
Gene ID 5978
NCBI Accession NP_005603
Pathways Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-REST Antibody Target Details REST Handling Images back to top
Application Notes Western Blotting: Approx 140 kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122 kDa according to NP_005603.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recomme

Peptide ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.

Approx 140 kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122 kDa according to NP_005603.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recomme
Restrictions For Research Use only

Handling

Product Details anti-REST Antibody Target Details REST Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.