anti-Human BCL2 antibody for Immunoprecipitation

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 41-54
162
74
65
62
44
29
25
25
24
21
17
15
15
15
11
10
10
10
10
9
7
7
7
7
7
6
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1068
263
220
52
26
22
19
16
5
4
3
3
3
2
2
2
Host
Mouse
578
556
18
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is conjugated to PE
52
51
44
41
35
25
24
16
15
15
14
14
14
14
14
14
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
847
419
384
364
340
338
233
194
168
81
40
15
14
13
12
10
9
3
3
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN638444
$ 417.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1302954 FACS ICC IHC (f) IHC (p) IP WB FITC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4
1 ABIN967311 IHC (fro) FACS IF IP WB Hamster IgG Log in to see 6C8 13
1 ABIN1302482 FACS ICC IHC (f) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4
1 ABIN222888 IP IHC WB Rabbit IgG N-Term Log in to see Polyclonal 4
1 ABIN967317 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
1 ABIN967318 IP WB Mouse IgG1 AA 61-76 Log in to see 4D7 10
1 ABIN967312 IHC (fro) FACS IF IP WB Armenian Hamster IgG Log in to see 6C8 13
1 ABIN1981878 IHC (f) ICC FACS IP IHC (p) WB APC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 3
1 ABIN2705576 IP IHC WB Rabbit pSer70 Log in to see Polyclonal 0
1 ABIN2862602 IP IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal 0
1 ABIN2862600 IF IP IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal 0
1 ABIN1531786 ELISA IHC IP WB Rabbit IgG AA 41-90, pSer70 Log in to see Polyclonal 1
1 ABIN1531787 IF IHC IP WB Rabbit IgG AA 26-75, pThr56 Log in to see Polyclonal 1
1 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
1 ABIN4283360 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 6
1 ABIN4283367 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN4283369 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
1 ABIN1876758 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN222984 IP IHC WB Mouse IgG1 Log in to see Bcl-2-100 6

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(162), (74), (65), (62), (44), (29), (25), (25), (24), (21), (17), (15), (15), (15), (11), (10), (10), (10), (10), (9), (7), (7), (7), (7), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1068), (263), (220), (52), (26), (22), (19), (16), (5), (4), (3), (3), (3), (2), (2), (2)
Host Mouse
(578), (556), (18), (6), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is conjugated to PE
(52), (51), (44), (41), (35), (25), (24), (16), (15), (15), (14), (14), (14), (14), (14), (14), (13), (13), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(847), (419), (384), (364), (340), (338), (233), (194), (168), (81), (40), (15), (14), (13), (12), (10), (9), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN638444) Images
Specificity The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
No Cross-Reactivity Mouse (Murine)
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Clone Bcl-2-100
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN638444) Images back to top
Antigen
Alternative Name BCL2 (BCL2 Antibody Abstract)
Background Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.
Gene ID 596
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN638444) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN638444) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-BCL2 antibody (ABIN638444)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Joubert, Marais, Maritz: "Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 33, Issue 1, pp. 67-70, 2009

Laforge, Petit, Estaquier, Senik: "Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef." in: Journal of virology, Vol. 81, Issue 20, pp. 11426-40, 2007

Gugasyan, Christou, OReilly, Strasser, Gerondakis: "Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA." in: Cell death and differentiation, Vol. 13, Issue 7, pp. 1235-7, 2006

Laflamme, Israël-Assayag, Cormier: "Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis." in: The European respiratory journal, Vol. 21, Issue 2, pp. 225-31, 2003

Soilu-Hänninen, Ekert, Bucci, Syroid, Bartlett, Kilpatrick: "Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 12, pp. 4828-38, 1999