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|Antigen||B-Cell CLL/lymphoma 2 (BCL2) Antibodies|
|Epitope||AA 41-54 Alternatives|
|Conjugate||This BCL2 antibody is conjugated to APC Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
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Product Details anti-BCL2 AntibodyTarget Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN1981878) Images
|Specificity||The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.|
|No Cross-Reactivity||Mouse (Murine)|
|Characteristics||The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.|
|Immunogen||Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2|
|Plasmids, Primers & others|
Target Details BCL2Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN1981878) Images back to top
|Alternative Name||Bcl2 (BCL2 Antibody Abstract)|
|Background||Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.|
|Pathways||MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN1981878) Images back to top
|Application Notes||The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN1981878) Images back to top
|Buffer||Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
|Storage Comment||Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.|
References for anti-BCL2 antibody (ABIN1981878)Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
|Product cited in:||
Joubert, Marais, Maritz: "Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 33, Issue 1, pp. 67-70, 2009
Gugasyan, Christou, OReilly, Strasser, Gerondakis: "Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA." in: Cell death and differentiation, Vol. 13, Issue 7, pp. 1235-7, 2006
Laflamme, Israël-Assayag, Cormier: "Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis." in: The European respiratory journal, Vol. 21, Issue 2, pp. 225-31, 2003
ImagesProduct Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN1981878) back to top