anti-Human BCL2 antibody for Immunoprecipitation

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 41-54
246
80
64
44
40
29
28
27
21
17
15
15
15
11
10
10
10
9
9
9
8
7
7
7
7
6
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1237
267
254
68
38
21
19
17
6
4
3
3
3
2
2
Host
Mouse
667
641
19
6
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is conjugated to APC
61
55
48
46
35
23
21
21
18
11
11
11
11
10
10
10
10
10
10
9
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
987
441
441
441
381
356
209
154
142
125
91
42
33
15
13
9
3
3
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN1981878
$ 566.06
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.752602 ABIN1302954 FACS ICC IHC (f) IHC (p) IP WB FITC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4
19.252602 ABIN3181930 ELISA IHC IP WB Rabbit IgG pSer70 Log in to see Polyclonal 0
19 ABIN1876758 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
17.752602 ABIN638444 FACS ICC IHC (f) IHC (p) IP WB PE Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 5
17.752602 ABIN1302482 FACS ICC IHC (f) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4
16.252602 ABIN3181931 ELISA IF IHC IP WB Rabbit IgG pThr56 Log in to see Polyclonal 0
15.979721 ABIN4283360 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 6
13.252602 ABIN5618921 IHC IP WB Rabbit Center Log in to see Polyclonal 0
13.252602 ABIN2134576 IP ELISA WB Mouse IgG2a kappa Log in to see 11C5 0
13.252602 ABIN2134575 IP ELISA WB Mouse IgG1 kappa Log in to see 2B11 0
13.252602 ABIN2134574 IP IHC WB Mouse IgG1 Log in to see Bcl-2-100 0
13.252602 ABIN2134581 IP Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.252602 ABIN2134580 IP IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
13.252602 ABIN1690611 IP Sepharose Mouse IgG1 Log in to see BL-2 0
13.252602 ABIN1690264 IP Magnetic Particles Mouse IgG1 Log in to see BL-2 0
11.5 ABIN543402 FACS IP ELISA WB Rabbit Log in to see Polyclonal 2
10 ABIN1531787 IF IHC IP WB Rabbit IgG AA 26-75, pThr56 Log in to see Polyclonal 1
10 ABIN6256408 ELISA ICC IF IHC IP WB Rabbit IgG pSer70 Log in to see Polyclonal 0
10 ABIN2888370 IF IHC IHC (p) IP WB Rabbit IgG pThr56 Log in to see Polyclonal 0
10 ABIN3026609 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa Log in to see 100-D5 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(246), (80), (64), (44), (40), (29), (28), (27), (21), (17), (15), (15), (15), (11), (10), (10), (10), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1237), (267), (254), (68), (38), (21), (19), (17), (6), (4), (3), (3), (3), (2), (2)
Host Mouse
(667), (641), (19), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is conjugated to APC
(61), (55), (48), (46), (35), (23), (21), (21), (18), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(987), (441), (441), (441), (381), (356), (209), (154), (142), (125), (91), (42), (33), (15), (13), (9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN1981878) Images
Specificity The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
No Cross-Reactivity Mouse (Murine)
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Clone Bcl-2-100
Isotype IgG1
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN1981878) Images back to top
Antigen
Alternative Name Bcl2 (BCL2 Antibody Abstract)
Background Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.
Gene ID 596
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN1981878) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN1981878) Images back to top
Buffer Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-BCL2 antibody (ABIN1981878)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Joubert, Marais, Maritz: "Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 33, Issue 1, pp. 67-70, 2009

Gugasyan, Christou, OReilly, Strasser, Gerondakis: "Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA." in: Cell death and differentiation, Vol. 13, Issue 7, pp. 1235-7, 2006

Laflamme, Israël-Assayag, Cormier: "Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis." in: The European respiratory journal, Vol. 21, Issue 2, pp. 225-31, 2003

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN1981878) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (APC) (ABIN1981878) Intracellular staining of human peripheral blood with anti-Bcl2 (Bcl-2/100) APC.