anti-Human BCL2 antibody for Immunoprecipitation

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
N-Term
251
79
64
44
40
29
28
27
21
17
15
15
15
10
10
10
10
9
9
9
8
7
7
7
7
6
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1222
264
241
70
38
21
19
19
6
4
3
3
3
2
2
Host
Rabbit
644
643
18
6
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
60
54
47
46
36
23
21
20
18
11
11
11
11
10
10
10
10
10
10
10
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
973
446
445
441
380
357
205
155
148
125
81
42
33
15
13
9
6
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN222888
$ 275.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN638444 FACS ICC IHC (f) IHC (p) IP WB PE Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 5
1 ABIN1302954 FACS ICC IHC (f) IHC (p) IP WB FITC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4
1 ABIN1981878 IHC (f) ICC FACS IP IHC (p) WB APC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 3
1 ABIN1302482 FACS ICC IHC (f) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4
1 ABIN2705576 IP IHC WB Rabbit pSer70 Log in to see Polyclonal 0
1 ABIN2862602 IP IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal 0
1 ABIN2862600 IF IP IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal 0
1 ABIN1531786 ELISA IHC IP WB Rabbit IgG AA 41-90, pSer70 Log in to see Polyclonal 1
1 ABIN1531787 IF IHC IP WB Rabbit IgG AA 26-75, pThr56 Log in to see Polyclonal 1
1 ABIN4283360 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 6
1 ABIN4283367 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN4283369 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
1 ABIN1876758 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN3181930 ELISA IHC IP WB Rabbit IgG pSer70 Log in to see Polyclonal 0
1 ABIN3181931 ELISA IF IHC IP WB Rabbit IgG pThr56 Log in to see Polyclonal 0
1 ABIN222984 IP IHC WB Mouse IgG1 Log in to see Bcl-2-100 6
1 ABIN2657117 FACS IP Alexa Fluor 647 Mouse IgG1 AA 41-54 Log in to see 100 7
1 ABIN5949366 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-200 Log in to see SZ10-03 0
1 ABIN2664061 FACS IP Mouse IgG1 AA 41-54 Log in to see 100 7
1 ABIN356812 EIA IHC (p) IP WB Rabbit Ig Fraction BH3 Domain Log in to see Polyclonal 1

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term
(251), (79), (64), (44), (40), (29), (28), (27), (21), (17), (15), (15), (15), (10), (10), (10), (10), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1222), (264), (241), (70), (38), (21), (19), (19), (6), (4), (3), (3), (3), (2), (2)
Host Rabbit
(644), (643), (18), (6), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(60), (54), (47), (46), (36), (23), (21), (20), (18), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (10), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(973), (446), (445), (441), (380), (357), (205), (155), (148), (125), (81), (42), (33), (15), (13), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN222888) Images
Blocking Peptide Blocking peptide for this product available: ABIN412606
Specificity Detects a 25 kDa protein, corresponding to the apparent molecular weight of the ? isoform of Bcl-2 on SDS-PAGE immunoblots.
Purification Affinity purified
Immunogen Synthetic peptide corresponding to the N-terminus of human Bcl-2
Isotype IgG
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN222888) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis.
Synonyms: BCL2, BCL 2
Gene ID 596
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN222888) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipation (4-8 μg/mL), and Immunohistochemistry (10-20 μg/mL). Detects a 25 kDa protein, corresponding to the apparent molecular weight of the ? isoform of Bcl-2 on SDS-PAGE immunoblots.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN222888) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Bcl-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BCL2 antibody (ABIN222888)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Eslami, Sharifi, Rahimi, Rahati: "Protective effect of telmisartan against oxidative damage induced by high glucose in neuronal PC12 cell." in: Neuroscience letters, 2013

Guérin, Cornut-Thibaut, Giscard-Destaing, Pouvreau, Guillemin, Aouacheria: "Subcellular dynamics of the maternal cell death regulator BCL2L10 in human preimplantation embryos." in: Human reproduction (Oxford, England), Vol. 28, Issue 3, pp. 729-39, 2013

Kiran Kumar, Prabhakara Rao, Noble, Weddington, McDowell, Rajanna, Bettaiya: "Lead-induced alteration of apoptotic proteins in different regions of adult rat brain." in: Toxicology letters, Vol. 184, Issue 1, pp. 56-60, 2008

Giuliani, Mioni, Altavilla, Leone, Bazzani, Minutoli, Bitto, Cainazzo, Marini, Zaffe, Botticelli, Pizzala, Savio, Necchi, Schiöth, Bertolini, Squadrito, Guarini: "Both early and delayed treatment with melanocortin 4 receptor-stimulating melanocortins produces neuroprotection in cerebral ischemia." in: Endocrinology, Vol. 147, Issue 3, pp. 1126-35, 2006

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN222888) back to top
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (N-Term) antibody (ABIN222888) Western blot analysis of Bcl-2 expression in HL60 cell lysate.