anti-Human Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like antibody for Enzyme Immunoassay

Recommended Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like Antibody (supplied by: Log in to see )

Antigen
Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) Antibodies
  • NEDD4-2
  • NEDD4.2
  • RSP5
  • hNEDD4-2
  • nedd4
  • nedd4-2
  • NEDD4
  • Nedd4-2
  • 1300012C07Rik
  • Nedd4b
  • neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
  • NEDD4L
  • neural precursor cell expressed, developmentally down-regulated gene 4-like
  • NEDD4L
  • LOC776799
  • nedd4l
  • Nedd4l
Epitope
AA 575-604, Middle Region
15
10
9
8
7
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
51
49
31
6
5
5
5
4
3
1
1
1
1
1
Host
Rabbit
59
7
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
44
23
13
7
7
5
4
2
1
Supplier
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Catalog No. ABIN953641
$ 390.50
Plus shipping costs $45.00

General

Antigen Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) Antibodies
Epitope AA 575-604, Middle Region
(15), (10), (9), (8), (7), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(51), (49), (31), (6), (5), (5), (5), (4), (3), (1), (1), (1), (1), (1)
Host Rabbit
(59), (7)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(44), (23), (13), (7), (7), (5), (4), (2), (1)
Pubmed 3 references available
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Product details

Target details Application Details Handling References Images
Specificity This antibody recognizes Human and Mouse NEDD4L (Center).
Purification Protein A column, followed by peptide affinity purification
Immunogen KLH conjugated synthetic peptide between 575-604 amino acids from the Central region of human NEDD4L
Isotype Ig

Target details

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Antigen
Alternative Name NEDD4L (NEDD4L Antibody Abstract)
Background E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK.Synonyms: KIAA0439, NEDD4.2, NEDL3, Nedd4-2
Molecular Weight 111932 Da
Gene ID 23327
NCBI Accession NP_001138436
Research Area Enzymes
Pathways Negative Regulation of Transporter Activity

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References

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Background publications

Zhou, Kabra, Olson, Piper, Snyder: "Hrs controls sorting of the epithelial Na+ channel between endosomal degradation and recycling pathways." in: The Journal of biological chemistry, Vol. 285, Issue 40, pp. 30523-30, 2010

Jin, Hong, Lim, Hwang, Lee, Shin, Park, Oh: "Genetic variations in the sodium balance-regulating genes ENaC, NEDD4L, NDFIP2 and USP2 influence blood pressure and hypertension." in: Kidney & blood pressure research, Vol. 33, Issue 1, pp. 15-23, 2010

Hanna, Kruskal, Ezekowitz, Bloom, Collier: "Role of macrophage oxidative burst in the action of anthrax lethal toxin." in: Molecular medicine (Cambridge, Mass.), Vol. 1, Issue 1, pp. 7-18, 1996

Images

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Supplier Images
Western Blotting (WB) image for anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) (AA 575-604), (Middle Region) antibody (ABIN953641) Western blot analysis of NEDD4L Antibody (Center) in mouse brain tissue lysates (35ug...