anti-Human Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like antibody for Western Blotting

Recommended Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like Antibody (supplied by: Log in to see )

Antigen
Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) Antibodies
  • NEDD4-2
  • NEDD4.2
  • RSP5
  • hNEDD4-2
  • nedd4
  • nedd4-2
  • NEDD4
  • Nedd4-2
  • 1300012C07Rik
  • Nedd4b
  • neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
  • NEDD4L
  • neural precursor cell expressed, developmentally down-regulated gene 4-like
  • NEDD4L
  • LOC776799
  • nedd4l
  • Nedd4l
Epitope
Middle Region
15
10
9
9
7
3
2
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
51
49
30
5
4
4
4
4
2
1
1
1
1
Host
Rabbit
59
7
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
44
23
13
7
7
5
4
2
1
1
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Catalog No. ABIN2774731
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1474795 ABIN953641 EIA WB Rabbit Ig AA 575-604, Middle Region Log in to see Polyclonal 3
3.1474795 ABIN2737237 WB Rabbit IgG Log in to see Polyclonal
3.1474795 ABIN631206 WB Rabbit Middle Region Log in to see Polyclonal
3.1474795 ABIN4890448 WB Rabbit Log in to see Polyclonal
3.1474795 ABIN525093 IP ELISA WB Mouse IgG2a kappa AA 1-100, partial Log in to see 1D2
3.1474795 ABIN525092 WB Mouse AA 1-911, full length Log in to see Polyclonal
1 ABIN468854 WB Rabbit AA 431-480 Log in to see Polyclonal
1 ABIN744257 IHC (p) WB HRP Rabbit IgG pSer342 Log in to see Polyclonal
1 ABIN5661405 IHC WB Rabbit Log in to see Polyclonal
1 ABIN1327618 ELISA WB Mouse AA 1-100, partial Log in to see Polyclonal
1 ABIN744250 IHC (p) WB Biotin Rabbit IgG pSer342 Log in to see Polyclonal
1 ABIN744248 IF (p) IHC (p) WB Rabbit IgG pSer342 Log in to see Polyclonal
1 ABIN2585867 ELISA WB Rabbit IgG AA 575-604 Log in to see Polyclonal
1 ABIN2625989 IF IHC (p) IP WB Rabbit IgG AA 225-275 Log in to see Polyclonal
1 ABIN2625990 IF IHC (p) IP WB Rabbit IgG AA 275-325 Log in to see Polyclonal
1 ABIN1916659 IP WB Rabbit IgG AA 425-475 Log in to see Polyclonal
1 ABIN1811841 WB Rabbit AA 576-604 Log in to see Polyclonal
1 ABIN361557 WB Mouse Log in to see Polyclonal 3
1 ABIN2319982 ELISA WB Mouse IgG2a kappa AA 1-100 Log in to see 1D2
1 ABIN1089300 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) Antibodies
Epitope Middle Region
(15), (10), (9), (9), (7), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(51), (49), (30), (5), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Rabbit
(59), (7)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(44), (23), (13), (7), (7), (5), (4), (2), (1), (1)
Pubmed 1 reference available
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Product details

Target details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN980594
Sequence TVTLSAPLEG AKDSPVRRAV KDTLSNPQSP QPSPYNSPKP QHKVTQSFLP
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against NEDD4L. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L

Target details

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Antigen
Alternative Name NEDD4L (NEDD4L Antibody Abstract)
Background NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
Alias Symbols: FLJ33870, KIAA0439, RSP5, hNedd4-2, NEDD4-2, NEDD4.2, hNEDD4-2
Protein Interaction Partner: NEDD4L, DAZAP2, UBE2D1, PTCH1, WNK3, TNK2, SNCA, SLC9A3, SMAD6, KCNH2, HCN1, SMAD3, UBE2N, SNA3, NOTCH1, UBE2D3, UBC, SGK1, KCNQ1, SGK3, GORAB, MED28, FBXL15, vpr, RNF11, AATF, YWHAQ, LAPTM5, SCNN1B, SMAD2, ARRDC3, ARRDC4, UBE2L3, UBE2D2, SMAD7, UBE2K, UB
Protein Size: 955
Molecular Weight 110 kDa
Gene ID 23327
NCBI Accession NM_015277, NP_056092
Research Area Enzymes
Pathways Negative Regulation of Transporter Activity

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 955 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Product cited in:

Raikwar, Thomas: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, 2008

Images

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Supplier Images
Western Blotting (WB) image for anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) (Middle Region) antibody (ABIN2774731) WB Suggested Anti-NEDD4L Antibody Titration: 0.2-1 ug/ml
ELISA Titer: