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The function and activity of a protein are often modulated by other proteins with which it interacts. Characterizing protein–protein interactions is critical to understand protein function and the biology of the cell. Quantitative affinity purification followed by mass spectrometry is a powerful approach to investigate physical interaction partners of a protein of interest. Recent advances in technology now enable a quantification of up to 100 interactomes per day.

GFP-Traps® (ChromoTek GmbH) are ideal for efficient, one-step isolation of fluorescent fusion proteins and their interacting partners. In combination with the in-StageTip (iST) Sample Preparation Kit (PreOmics GmbH) both ensure an ideal sample preparation with high reproducibility and efficiency. The GFP-Trap® is a monovalent matrix with GFP-binding proteins coupled to it. They are suited for efficient, one-step isolation of fluorescent fusion proteins and their interacting partners. The GFP-Traps® are available either coupled to agarose beads or magnetic beads. The in-StageTip method is able to perform sample processing, from cell lysis through elution of purified peptides, in a single, enclosed volume. This robust and scalable method largely eliminates contamination or loss.1

We tested PreOmics’ iST kit and were amazed by the results. The number of identified peptides & proteins was 20–30% higher compared to what is achieved using our standard lab procedure. On top of that, the iST kit and protocol are so convenient as it includes everything ready-to-use and allows you to save a lot of time. Using the iST kit enables you to start processing your samples in the morning and have them ready for LC-MS in the afternoon. — Dr. Manuel Tzouros (project leader), Discovery Proteomics, Roche Innovation Center Basel about Preomics

The DetectX® Ferric Reducing Antioxidant Power (FRAP™) Detection Kit is designed to quantitatively measure antioxidant status in a variety of samples. The assay measures the antioxidant ability from all species. A Ferrous Chloride standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve.