The antibody for the detection of Cadherin 5 (CDH5) has now been successfully validated for immunofluorescence. A clear signal of the calcium-depending adhesion molecule was achieved in murine liver tissue while murine brain tissue (known to be negative for cadherin 5) remained unstained.
Cadherin 5 (also called CD144) is a vascular endothelial cadherin. This type of cadherins appears at the contact site of endothelial cells. They are functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, and additional they fundction also as a desmosome.
In mid 2013 the "Independent Validation Initiative" started as a partnership between
Science Exchange and antibodies-online.com. The project aims to enable scientists to select products (presently antibodies or ELISA kits) of their interest to be validated by labs from top academic research institutes.
Since we started the initiative we have successfully validated 42 products. The first was a anti-Fibrinogen antibody, followed by a couple of other interesting antibodies and ELISA kits. The latest succesfully validated product is an antibody for the detection of Cadherin 5 (CDH5). A few more products are currentlly being validated.
If you are interested in having a product validated, you can simply click the "Request validation" button on the product site. When requesting a validation you can specify the application the product should be validated for. Validation is free for all customers and costs will be covered by the respective supplier.
Here is a full list of successfully validated products:
| Catalog No. |
Antigen Name |
Product Type |
Tested for |
| ABIN197433 |
Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) |
Rabbit IgG |
IF |
| ABIN707576 |
Aquaporin 2 (Collecting Duct) (AQP2) |
Rabbit IgG |
IHC |
| ABIN729343 |
Cadherin 5 (CDH5) |
Rabbit IgG |
IF |
| ABIN968006 |
Clathrin (AA 4-171,Heavy Chain) |
Mouse IgG1 |
IHC |
| ABIN708086 |
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) |
Rabbit IgG |
IHC |
| ABIN1112984 |
DYKDDDDK Tag |
Mouse IgG1 |
IF |
| ABIN734003 |
Elastin (ELN) (C-Term) |
Rabbit IgG |
IHC |
| ABIN673854 |
Fibrinogen |
Rabbit IgG |
ELISA |
| ABIN728713 |
NK2 Homeobox 1 (NKX2-1) (N-Term) |
Rabbit IgG |
WB |
| ABIN675024 |
Tight Junction Protein 1 (Zona Occludens 1) (TJP1) |
Rabbit IgG |
IHC |
| ABIN967416 |
Tumor Protein P53 (TP53) |
Rabbit IgG |
WB |
| ABIN364986 |
Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ) |
ELISA Kit |
ELISA |
| ABIN364989 |
alpha-Fetoprotein (AFP) |
Rabbit IgG |
WB |
| ABIN365222 |
Angiogenin, Ribonuclease, RNase A Family, 5 (ANG) |
ELISA Kit |
ELISA |
| ABIN810667 |
Angiopoietin 1 (ANGPT1) |
ELISA Kit |
ELISA |
| ABIN578045 |
Angiopoietin-Like 2 (ANGPTL2) (Human) |
ELISA Kit |
ELISA |
| ABIN366603 |
CD105 (Endoglin / TGF-Beta1/3 Receptor) |
ELISA Kit |
ELISA |
| ABIN1136417 |
Chromosome 19 Open Reading Frame 80 (C19ORF80) |
ELISA Kit |
ELISA |
| ABIN1000178 |
cortisol (COR) |
ELISA Kit |
ELISA |
| ABIN365082 |
Cystatin C (CST3) |
ELISA Kit |
ELISA |
| ABIN419782 |
Defensin, alpha 3, Neutrophil-Specific (DEFa3) |
ELISA Kit |
ELISA |
| ABIN1372755 |
Dehydroepiandrosterone Sulfate (Human) |
ELISA Kit |
ELISA |
| ABIN1372754 |
Dehydroepiandrosterone Sulfate (Rat) |
ELISA Kit |
ELISA |
| ABIN837930 |
Epidermal Growth Factor Receptor (Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog, Avian) (EGFR) |
ELISA Kit |
ELISA |
| ABIN1114880 |
Ferritin (FE) (Human) |
ELISA Kit |
ELISA |
| ABIN512884 |
Follicle-Stimulating Hormone (FSH) |
ELISA Kit |
ELISA |
| ABIN816582 |
Interferon Regulatory Factor 4 (IRF4) (Mouse) |
ELISA Kit |
ELISA |
| ABIN365163 |
IL-6 (Human) |
ELISA Kit |
ELISA |
| ABIN414887 |
Interleukin 8 (IL8) |
ELISA Kit |
ELISA |
| ABIN365215 |
Interleukin 18 (IL18) |
ELISA Kit |
ELISA |
| ABIN365165 |
Leptin (LEP) |
ELISA Kit |
ELISA |
| ABIN365998 |
Lipocalin 2 (LCN2) |
ELISA Kit |
ELISA |
| ABIN512262 |
Luteinizing hormone (LH) |
ELISA Kit |
ELISA |
| ABIN818177 |
Matrix Metalloproteinase 9 (MMP9) |
ELISA Kit |
ELISA |
| ABIN1690717 |
NGAL/MMP-9 complex |
ELISA Kit |
ELISA |
| ABIN368630 |
Pro-Brain Natriuretic Peptide (NT-ProBNP |
ELISA Kit |
ELISA |
| ABIN457074 |
Soluble Cluster of Differentiation 14 (sCD14) |
ELISA Kit |
ELISA |
| ABIN366828 |
Thrombospondin 1 (THBS1) |
ELISA Kit |
ELISA |
| ABIN510312 |
Thyroid Stimulating Hormone (TSH) (Human) |
ELISA Kit |
ELISA |
| ABIN365420 |
TIMP Metallopeptidase Inhibitor 1 (TIMP1) |
ELISA Kit |
ELISA |
| ABIN367608 |
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) |
ELISA Kit |
ELISA |
| ABIN365402 |
Transforming Growth Factor, beta 1 (TGFB1) |
ELISA Kit |
ELISA |
