Determining whether two proteins interact is a characteristically challenging endeavor, particularly in systems where low concentration makes traditional co-immunoprecipitation experiments nontrivial.
The proximity ligation assay (PLA) allows researchers to rapidly and simply determine whether two proteins interact.
The proximity ligation assay (PLA) is the result of a harmonious marriage between affinity proteomics and molecular biology techniques. The PLA employs a paired set of antibodies that are conjugated to special matching oligonucleotide probes. Simple, reliable rolling circle DNA amplification is leveraged to provide a readout that confirms protein interaction.
antibodies-online currently offers more than 1,500 reagents (747 individual antibodies and 766 verified antibody-pairs) for use in proximity ligation assay experiments.