D-dimers as indicator for the function of the coagulation system

D-Dimers are proteins, that occur as degradation products of cross-linked fibrin in the blood during endogenous dissolution of a blood clot and are caused by the reactive action of plasmin.

Coagulation occurs when the proteins of the coagulation cascade are activated, either by contact with damaged blood vessel wall and exposure to collagen in the tissue space (intrinsic pathway) or by activation of factor VII by tissue activating factors (extrinsic pathway).

In both ways thrombin is generated, an enzyme which is able to convert fibrinogen into the insoluble fibrin. Another thrombin-generated enzyme, factor XIII, then crosslinks the fibrin proteofibrils at the D fragment site, leading to the formation of an insoluble gel which serves as a scaffold for blood clot formation.

D-Dimer determination is an important instrument in the diagnosis of blood clotting related diseases. The D-Dimer concentration in blood is an indicator for formation of a blood clot inside a blood vessel, also called thrombosis. In addition, it is used in the diagnosis of the blood disorder disseminated intravascular coagulation.

coagulation system

The process of blood clotting is not yet fully understood which is why the research on D-Dimers still is off big relevance. The D-Dimer ELISA Kit from Abbexa is able to quantitatively determine the D-Dimer levels in murine plasma samples. The kit offers high sensitivity; it is able to differ between concentration levels of less than < 0.244 ng/mL. Another advantage is the low sample amount needed, only 0.5 µl of mouse plasma is sufficient for D-Dimer level determination.

Learn more about the D-Dimer ELISA kit!