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|Antigen||B-Cell CLL/lymphoma 3 (BCL3) Antibodies|
|Conjugate||This BCL3 antibody is un-conjugated Alternatives|
ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Product Details anti-BCL3 AntibodyTarget Details BCL3 Application Details Handling Images
|Purification||This affinity purified antibody is directed against human BCL3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.|
Immunogen: Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
|Plasmids, Primers & others|
Target Details BCL3Product Details anti-BCL3 Antibody Application Details Handling Images back to top
|Alternative Name||BCL3 (BCL3 Antibody Abstract)|
Synonyms: rabbit anti-BCL3 antibody, rabbit anti-BCL-3 Antibody, BCL 3, B-cell lymphoma 3 protein, B-cell lymphoma 3 protein Antibody
Background: BCL3 belongs to the ANKRD family. The protein contains seven ankyrin repeats, which are closely related to those found in I kappa B proteins. BCL3 functions as a transcriptional co-activator, regulator of transcriptional activation, promotor of transcription, and inhibitor of nuclear translocation of NF-kappa B. It is a proto-oncogene identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. BCL3 contributes to the regulation of cell proliferation. Therefore, BCL3 may be associated with B-cell chronic lymphocytic leukemia, lymphoma, or other hematologic diseases. Anti-BCL3 Antibody is useful for researchers interested in Cancer Research, NF-Kappa B Pathway, and TNF Signaling Pathway.
Gene Name: BCL3
|Pathways||NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus|
Application DetailsProduct Details anti-BCL3 Antibody Target Details BCL3 Handling Images back to top
Immunohistochemistry Dilution: 1:200
Application Note: Anti-BCL-3 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at 47.5 kDa in western blot using appropriate lysates. Positive control used recombinant BCL3 protein, HEK293T overexpressing BCL3 or U87 whole cell lysates at 1 μg/mL in WB, and U87MG with PFA at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 5.0 μg/mL
Flow Cytometry Dilution: User Optimized
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 10 μg/mL
|Restrictions||For Research Use only|
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||RT,4 °C,-20 °C|
|Storage Comment||Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
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