anti-Human BCL3 antibody for Fluorescence Microscopy

Recommended BCL3 Antibody

Antigen
B-Cell CLL/lymphoma 3 (BCL3) Antibodies
  • BCL3
  • AI528691
  • Bcl-3
  • BCL4
  • D19S37
  • B-cell CLL/lymphoma 3
  • B cell leukemia/lymphoma 3
  • BCL3
  • Bcl3
Epitope
C-Term
9
9
9
8
8
7
7
6
5
5
4
3
3
3
1
1
Reactivity
Human
118
86
25
1
1
1
1
1
1
1
Host
Rabbit
110
27
8
5
Clonality
Polyclonal
Conjugate
This BCL3 antibody is un-conjugated
13
9
7
6
4
4
4
4
4
4
3
3
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
101
51
40
23
18
17
13
13
12
3
3
2
1
1
1
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Available images

Catalog No. ABIN6655290
$ 394.90
Plus shipping costs $45.00 and $22.60 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13 ABIN6655291 ELISA FACS FM IHC IP WB Rabbit IgG C-Term Polyclonal 0

General

Antigen B-Cell CLL/lymphoma 3 (BCL3) Antibodies
Epitope C-Term
(9), (9), (9), (8), (8), (7), (7), (6), (5), (5), (4), (3), (3), (3), (1), (1)
Reactivity Human
(118), (86), (25), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(110), (27), (8), (5)
Clonality
Polyclonal
Conjugate This BCL3 antibody is un-conjugated
(13), (9), (7), (6), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(101), (51), (40), (23), (18), (17), (13), (13), (12), (3), (3), (2), (1), (1), (1)
Supplier

Product Details anti-BCL3 Antibody

Target Details BCL3 Application Details Handling Images
Purification This affinity purified antibody is directed against human BCL3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.
Immunogen

Immunogen: Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype IgG
Plasmids, Primers & others

Target Details BCL3

Product Details anti-BCL3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name BCL3 (BCL3 Antibody Abstract)
Background

Synonyms: rabbit anti-BCL3 antibody, rabbit anti-BCL-3 Antibody, BCL 3, B-cell lymphoma 3 protein, B-cell lymphoma 3 protein Antibody

Background: BCL3 belongs to the ANKRD family. The protein contains seven ankyrin repeats, which are closely related to those found in I kappa B proteins. BCL3 functions as a transcriptional co-activator, regulator of transcriptional activation, promotor of transcription, and inhibitor of nuclear translocation of NF-kappa B. It is a proto-oncogene identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. BCL3 contributes to the regulation of cell proliferation. Therefore, BCL3 may be associated with B-cell chronic lymphocytic leukemia, lymphoma, or other hematologic diseases. Anti-BCL3 Antibody is useful for researchers interested in Cancer Research, NF-Kappa B Pathway, and TNF Signaling Pathway.

Gene Name: BCL3

Gene ID 602
NCBI Accession NP_005169
UniProt P20749
Pathways NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus

Application Details

Product Details anti-BCL3 Antibody Target Details BCL3 Handling Images back to top
Application Notes

Immunohistochemistry Dilution: 1:200

Application Note: Anti-BCL-3 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at 47.5 kDa in western blot using appropriate lysates. Positive control used recombinant BCL3 protein, HEK293T overexpressing BCL3 or U87 whole cell lysates at 1 μg/mL in WB, and U87MG with PFA at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 5.0 μg/mL

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 10 μg/mL

Restrictions For Research Use only

Handling

Product Details anti-BCL3 Antibody Target Details BCL3 Application Details Images back to top
Format Liquid
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Images

Product Details anti-BCL3 Antibody Target Details BCL3 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-B-Cell CLL/lymphoma 3 (BCL3) (C-Term) antibody (ABIN6655290) Immunofluorescence microscopy of BCL3 Immunofluorescence microscopy of Anti-BCL3 in C...
Immunofluorescence (IF) image for anti-B-Cell CLL/lymphoma 3 (BCL3) (C-Term) antibody (ABIN6655290) Immunofluorescence Microscopy of Rabbit anti-BCL3 antibody Immunofluorescence Microsc...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 3 (BCL3) (C-Term) antibody (ABIN6655290) Western Blot of Rabbit anti-BCL3 antibody Western Blot of Rabbit anti-BCL3 antibody. ...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 3 (BCL3) (C-Term) antibody (ABIN6655290) Western Blot of Rabbit anti-BCL3 antibody Western Blot of Rabbit anti-BCL3 antibody. ...