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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN718541 | IF (p) IHC (p) WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
1 | ABIN127088 | IHC (p) IP WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
1 | ABIN718550 | IHC (p) WB | HRP | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
1 | ABIN718543 | IHC (p) WB | Biotin | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
1 | ABIN3030103 | IHC (p) WB | Rabbit | IgG | Middle Region | Log in to see | Polyclonal | 0 |
General |
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Antigen | B-Cell CLL/lymphoma 3 (BCL3) Antibodies |
Epitope | AA 199-216, Middle Region Alternatives |
Reactivity | Human, Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This BCL3 antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-BCL3 AntibodyTarget Details BCL3 Application Details Handling Images |
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Purpose | Rabbit IgG polyclonal antibody for B-cell lymphoma 3 protein(BCL3) detection. Tested with WB, IHC-P in Human,Mouse,Rat. |
Sequence | MALDRHGQTA AHLACEHR |
Cross-Reactivity (Details) |
Predicted Cross Reactivity: mouse No cross reactivity with other proteins. Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. |
Characteristics |
Rabbit IgG polyclonal antibody for B-cell lymphoma 3 protein(BCL3) detection. Tested with WB, IHC-P in Human,Mouse,Rat. Gene Name: B-cell CLL/lymphoma 3 Protein Name: B-cell lymphoma 3 protein(BCL-3) |
Purification | Immunogen affinity purified. |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Bcl3(199-216aa MALDRHGQTAAHLACEHR), different from the related rat and mouse sequences by one amino acid. |
Isotype | IgG |
Target Details BCL3Product Details anti-BCL3 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | BCL3 (BCL3 Antibody Abstract) |
Background |
B-cell lymphoma 3-encoded protein is a protein that in humans is encoded by the BCL3 gene. This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. The BCL3 gene is mapped to human chromosome 19. This protein functions as a transcriptional coactivator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. Synonyms: B cell CLL/lymphoma 3 antibody|B cell leukemia/lymphoma 3 antibody|B cell lymphoma 3 encoded protein antibody|B-cell lymphoma 3 protein antibody|Bcl 3 antibody|BCL 4 antibody|BCL-3 antibody|Bcl3 antibody|BCL3_HUMAN antibody|BCL4 antibody|Chronic lymphatic leukemia protein antibody|D19S37 antibody|Protein Bcl 3 antibody|Proto-oncogene BCL3 antibody |
UniProt | P20749 |
Pathways | NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus |
Application DetailsProduct Details anti-BCL3 Antibody Target Details BCL3 Handling Images back to top |
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Application Notes |
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Comment |
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P). |
Restrictions | For Research Use only |
HandlingProduct Details anti-BCL3 Antibody Target Details BCL3 Application Details Images back to top |
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Format | Lyophilized |
Reconstitution | Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL. |
Concentration | 500 μg/mL |
Buffer | Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide. |
Preservative | Thimerosal (Merthiolate), Sodium azide |
Precaution of Use | This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. |
Expiry Date | 12 months |
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Supplier Images |