anti-Human CD40 antibody for Immunofluorescence (fixed cells)

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Epitope
AA 1-193
53
41
37
24
23
22
19
18
11
7
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
637
312
68
20
14
10
10
6
4
3
2
1
1
1
1
1
Host
Mouse
496
214
167
22
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
86
84
48
36
24
21
17
15
13
13
9
9
9
9
9
9
9
8
7
7
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
577
244
214
189
109
103
96
63
50
33
19
14
13
13
9
6
6
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1997455
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1997449 FACS IF IF/ICC Mouse AA 1-193 Log in to see 03 0
1 ABIN1843091 ELISA IF/ICC Mouse IgG1 Log in to see 0
1 ABIN3037248 FACS IF/ICC PE Mouse IgG2b Log in to see 0
1 ABIN3037247 FACS IF/ICC FITC Mouse IgG2b Log in to see 0
1 ABIN3037249 FACS IF/ICC PE-Cy5 Mouse IgG2b Log in to see 0
1 ABIN3071431 FACS IF/ICC Mouse IgG2b Log in to see 0
1 ABIN2900390 FACS IF/ICC Mouse IgG1 Log in to see 0
1 ABIN1853654 FACS IF/ICC WB FITC Mouse IgG1 Log in to see 0
1 ABIN1853655 FACS IF/ICC WB PE Mouse IgG1 Log in to see 0
1 ABIN1853656 FACS IF/ICC WB APC Mouse IgG1 Log in to see 0
1 ABIN1853657 FACS IF/ICC WB PE-Cy5 Mouse IgG1 Log in to see 0
1 ABIN2994257 IF/ICC IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN2895750 ELISA IF/ICC IHC WB Rabbit AA 26-187 Log in to see Polyclonal 0

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 1-193
(53), (41), (37), (24), (23), (22), (19), (18), (11), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(637), (312), (68), (20), (14), (10), (10), (6), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(496), (214), (167), (22), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(86), (84), (48), (36), (24), (21), (17), (15), (13), (13), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(577), (244), (214), (189), (109), (103), (96), (63), (50), (33), (19), (14), (13), (13), (9), (6), (6), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling Images
Specificity Human CD40 / TNFRSF5
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD40 / TNFRSF5 (rh CD40 / TNFRSF5, ABIN2003380 NP_001241.1, Met1-Arg193,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human CD40 / TNFRSF5 protein (ABIN2003380)
Clone 17
Isotype IgG1

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The cognate interaction between CD40 and CD40 ligand (CD154) on T cells activates NF-kappa B, Jun N-terminal kinase, and Janus kinase signal transducers and activators of transcription pathways. Several different TRAF proteins (Adaptor proteins) have been identified to serves as mediators of the signal transduction. In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
NCBI Accession NP_001241.1
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling Images back to top
Application Notes ICC/IF: 10-25 μg/mL
FCM: 0.5-2 μg/Test
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 1-193) antibody (ABIN1997455) Immunofluorescence staining of Human CD40 in Hep G2 cells. Cells were fixed with 4% P...