|Antigen||CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies|
|Conjugate||This CD40 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA)
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Product Details anti-CD40 AntibodyTarget Details CD40 Application Details Handling Images
|Specificity||CD40 is a 49 kDa type I integral membrane protein of the NGF receptor family. CD40 is found on normal and neoplastic B cells.(1) It is a pan-B marker, absent only from terminally differentiated plasma cells. CD40 is predicted to function in the process of B cell selection in the germinal center. Studies demonstrated that the antibody induces strong homotypic adhesions in resting B cells and, together with Interleukin-4 (IL-4) maintains the cell cycle of B blasts and promotes the switch to IgE secretion. (1,2) Purified B cells cultured in the CD40 system in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. (3) The ligand for CD40 is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion. (4) This clone acts in synergy with IL-4 to induce the increase of B-cell volume and the proliferation of anti IgM antibody-co-stimulated or pre-activated B cells. (5)|
|Characteristics||MAb to CD40 B cells, Monoclonal antibody to CD40 B cells|
|Purification||Protein A Chromatography|
|Immunogen||Anti-mu activated tonsillar human B cells|
Target Details CD40Product Details anti-CD40 Antibody Application Details Handling Images back to top
|Alternative Name||CD40 B Cells (CD40 Antibody Abstract)|
|Pathways||NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD40 Antibody Target Details CD40 Handling Images back to top
|Application Notes||Flow cytometry Study of B-cell proliferation, B-cell growth and activation factors Functional study of B-cell subpopulations. Fluorescent microscopy or flow cytometry: 2 µg/5x105 cells/test Activation assays: Working concentration: 10 ng/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Antibody Target Details CD40 Application Details Images back to top
|Reconstitution||Reconstitute with 1 mL of distilled water.|
|Buffer||Lyophilized from PBS containing 1 mg/mL BSA|
Avoid multiple freeze/thaw cycles. The addition of 0.1 % (w/v) sodium azide is recommended for storage of the Reconstituted form for up to one month at 2-8 °C.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
|Storage||4 °C/-20 °C|
|Storage Comment||Store lyophilized product at 2-8 °C. After reconstitution, aliquot and store at -28 °C.|