anti-Mouse (Murine) CD40 antibody for Intracellular Flow Cytometry

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • CD40 antigen
  • CD40
  • Cd40
Reactivity
Mouse (Murine)
629
277
75
20
15
10
9
6
4
4
3
2
2
1
1
1
1
1
Host
Hamster
476
190
168
21
6
2
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
82
80
46
36
18
15
12
11
10
9
7
6
6
6
6
6
6
6
6
6
6
5
5
4
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
539
254
206
182
109
92
83
81
50
32
20
18
13
9
6
6
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2665701
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN2658659 ICFC APC Rat IgG2a kappa Log in to see 3-23 4
4 ABIN2665993 IF ICFC IP IHC WB Rat IgG2a kappa Log in to see 3-23 3
4 ABIN2665696 ICFC Rat IgG2a kappa Log in to see 3-23 3
4 ABIN2660110 ICFC PerCP-Cy5.5 Rat IgG2a kappa Log in to see 3-23 3
4 ABIN2657785 ICFC Alexa Fluor 647 Rat IgG2a kappa Log in to see 3-23 3
1 ABIN2665700 ICFC Hamster IgM kappa Log in to see HM40-3 11
1 ABIN2658660 ICFC APC Rat IgG2a kappa Log in to see 3-23 4
1 ABIN2665697 ICFC Rat IgG2a kappa Log in to see 3-23 3
1 ABIN2657786 ICFC Alexa Fluor 647 Rat IgG2a kappa Log in to see 3-23 3
1 ABIN2660111 ICFC PerCP-Cy5.5 Rat IgG2a kappa Log in to see 3-23 3

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Mouse (Murine)
(629), (277), (75), (20), (15), (10), (9), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Hamster
(476), (190), (168), (21), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(82), (80), (46), (36), (18), (15), (12), (11), (10), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(539), (254), (206), (182), (109), (92), (83), (81), (50), (32), (20), (18), (13), (9), (6), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2665701) Images
Brand LEAF™
Purification The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level

Endotoxin level is <0.1 EU/μg of the protein (<0.01 ng/μg of the protein) as determined by the LAL test.

Clone HM40-3
Isotype IgM kappa
Plasmids, Primers & others

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN2665701) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN2665701) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN2665701) Images back to top
Concentration 1.0 mg/mL
Preservative Azide free
Handling Advice This LEAF™ solution contains no preservative, handle under aseptic conditions
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD40 antibody (ABIN2665701)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Ma, Xu, An, Dong, Lin, Shi, Wu: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006

Yu, Siegel, Roeder: "Interaction of the B cell-specific transcriptional coactivator OCA-B and galectin-1 and a possible role in regulating BCR-mediated B cell proliferation." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15505-16, 2006

Morelli, OConnell, Khanna, Logar, Lu, Thomson: "Preferential induction of Th1 responses by functionally mature hepatic (CD8alpha- and CD8alpha+) dendritic cells: association with conversion from liver transplant tolerance to acute rejection." in: Transplantation, Vol. 69, Issue 12, pp. 2647-57, 2000

Akiba, Oshima, Takeda, Atsuta, Nakano, Nakajima, Nohara, Yagita, Okumura: "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7058-66, 1999

Ridge, Di Rosa, Matzinger: "A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell." in: Nature, Vol. 393, Issue 6684, pp. 474-8, 1998

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, 1997

Kaneko, Hirose, Abe, Yagita, Okumura, Shirai: "CD40-mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus." in: European journal of immunology, Vol. 26, Issue 12, pp. 3061-5, 1997

Kashiwada, Kaneko, Yagita, Okumura, Takemori: "Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells." in: European journal of immunology, Vol. 26, Issue 7, pp. 1451-8, 1996

Inaba, Inaba, Fukuba, Mori, Haruna, Doi, Adachi, Iwai, Hosaka, Hisha: "Activation of thymic B cells by signals of CD40 molecules plus interleukin-10." in: European journal of immunology, Vol. 25, Issue 5, pp. 1244-8, 1995

Banchereau, Bazan, Blanchard, Brière, Galizzi, van Kooten, Liu, Rousset, Saeland: "The CD40 antigen and its ligand." in: Annual review of immunology, Vol. 12, pp. 881-922, 1994

Clark, Ledbetter: "Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 12, pp. 4494-8, 1986

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN2665701) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody (ABIN2665701) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody