anti-Non-Human Primate CD40 Ligand antibody for Immunocytochemistry

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
Reactivity
Human, Non-Human Primate
455
196
63
33
20
12
8
5
4
3
3
3
3
3
3
2
2
1
1
1
Host
Mouse
255
207
86
31
25
17
3
1
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is conjugated to PE
76
58
41
15
7
7
7
6
6
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS)
346
298
153
108
71
42
40
37
34
23
13
12
9
9
8
6
5
4
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN2749175
$ 506.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.75568 ABIN2752002 Func ICC FACS Mouse IgG1 Log in to see 24-31 7
17.25568 ABIN2749183 Func ICC FACS Mouse IgG1 Log in to see 24-31 5
15.755681 ABIN5710572 FACS Func ICC FITC Mouse IgG1 Log in to see 24-31 0

General

Antigen CD40 Ligand (CD40LG) Antibodies
Reactivity Human, Non-Human Primate
(455), (196), (63), (33), (20), (12), (8), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(255), (207), (86), (31), (25), (17), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is conjugated to PE
(76), (58), (41), (15), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS)
(346), (298), (153), (108), (71), (42), (40), (37), (34), (23), (13), (12), (9), (9), (8), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand antibody (ABIN2749175) Images
Specificity The mouse monoclonal antibody 24-31 detects CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen human CD154 fusion protein
Clone 24-31
Isotype IgG1
Plasmids, Primers & others

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand antibody (ABIN2749175) Images back to top
Antigen
Alternative Name CD154 / CD40L (CD40LG Antibody Abstract)
Background CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Gene ID 959
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand antibody (ABIN2749175) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand antibody (ABIN2749175) Images back to top
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD40 Ligand antibody (ABIN2749175)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Product cited in:

Fernandes, Gomes, Butcher, Hernandez-Alcoceba, Chang, Kansopon, Newman, Stone, Tong: "Growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 ligand transgene." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 15, pp. 4847-56, 2009

Kum, Hung, Cameron, Chow et al.: "Temporal sequence and functional implications of V beta-specific T cell receptor down-regulation and costimulatory molecule expression following in vitro stimulation with the staphylococcal ..." in: The Journal of infectious diseases, Vol. 185, Issue 4, pp. 555-60, 2002

Brams, Black, Padlan, Hariharan, Leonard, Chambers-Slater, Noelle, Newman: "A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation." in: International immunopharmacology, Vol. 1, Issue 2, pp. 277-94, 2001

Barnhart, Ford, Bhushan, Song, Covey: "A polymorphic CD40 ligand (CD154) molecule mediates CD40-dependent signalling but interferes with the ability of soluble CD40 to functionally block CD154:CD40 interactions." in: Immunology, Vol. 99, Issue 1, pp. 54-61, 2000

Berner, Wolf, Hummel, Müller, Reuss-Borst: "Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis." in: Annals of the rheumatic diseases, Vol. 59, Issue 3, pp. 190-5, 2000

Grammer, McFarland, Heaney, Darnell, Lipsky: "Expression, regulation, and function of B cell-expressed CD154 in germinal centers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4150-9, 1999

Kornbluth, Kee, Richman: "CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive beta-chemokines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5205-10, 1998

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand antibody (ABIN2749175) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Ligand (CD40LG) antibody (PE) (ABIN2749175) Surface staining of PMA/ionomycin-activated PBMC using anti-CD154 (24-31) PE.