We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Epidermal Growth Factor (EGF) Antibodies|
|Conjugate||This EGF antibody is un-conjugated Alternatives|
Immunofluorescence (IF), ELISA, Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling References for anti-EGF antibody (ABIN966030) Images
|Immunogen||Purified recombinant fragment of human EGF expressed in E. Coli.|
|Plasmids, Primers & others|
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling References for anti-EGF antibody (ABIN966030) Images back to top
|Alternative Name||EGF (EGF Antibody Abstract)|
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Synonyms: URG, HOMG4, EGF
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation|
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF antibody (ABIN966030) Images back to top
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF antibody (ABIN966030) Images back to top
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
References for anti-EGF antibody (ABIN966030)Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
|Product cited in:||
Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003
Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002
Gual, Giordano, Williams, Rocchi, Van Obberghen, Comoglio: "Sustained recruitment of phospholipase C-gamma to Gab1 is required for HGF-induced branching tubulogenesis." in: Oncogene, Vol. 19, Issue 12, pp. 1509-18, 2000
Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993