anti-Human EGF antibody for Immunoprecipitation

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Epitope
AA 648-1186
25
18
17
16
12
12
12
11
10
9
8
8
7
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
381
186
109
20
17
9
4
2
2
1
1
1
Host
Mouse
370
147
32
26
6
3
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
57
29
23
11
11
9
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
417
282
159
101
54
33
30
29
27
26
23
15
14
7
7
5
4
3
2
1
1
Supplier
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Catalog No. ABIN1449300
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5947971 IHC IHC (p) IP WB Rabbit IgG AA 300-500 Log in to see JA14-15 0
1 ABIN1825592 IP IHC ELISA WB Mouse IgG2b Log in to see NYRhEGF 0
1 ABIN6252162 IP WB Mouse IgG1 Log in to see H9B4 0
1 ABIN1818535 ICC FACS IP WB Mouse IgG2b, kappa AA 648-1186 Log in to see 6F1 0
1 ABIN226930 IP ELISA WB Mouse IgG2b Log in to see 0
1 ABIN2927952 IF/ICC IHC IP WB Rabbit AA 971-1023 Log in to see Polyclonal 0
1 ABIN2927951 IF/ICC IHC IP WB Rabbit AA 609-751 Log in to see Polyclonal 0
1 ABIN6002743 IF/ICC IHC IP WB Mouse AA 609-751 Log in to see 0
1 ABIN2770965 ELISA IHC (p) IP WB Rabbit AA 1166-1180 Log in to see Polyclonal 0
1 ABIN2260982 IP ELISA WB Mouse IgG2b Log in to see 5C4 0
1 ABIN2139661 IP IHC ELISA WB Mouse IgG2b Log in to see NYRhEGF 0
1 ABIN645596 ICC IP ELISA WB Mouse IgG2b Log in to see 0
1 ABIN1002258 IP ELISA WB Mouse IgG2b Log in to see NYRhEGF 0
1 ABIN6022728 IF/ICC IHC IP WB Mouse Log in to see 0
1 ABIN808209 IP TCC ELISA Mouse Log in to see NYRhEGF 0

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 648-1186
(25), (18), (17), (16), (12), (12), (12), (11), (10), (9), (8), (8), (7), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(381), (186), (109), (20), (17), (9), (4), (2), (2), (1), (1), (1)
Host Mouse
(370), (147), (32), (26), (6), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(57), (29), (23), (11), (11), (9), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(417), (282), (159), (101), (54), (33), (30), (29), (27), (26), (23), (15), (14), (7), (7), (5), (4), (3), (2), (1), (1)
Pubmed 9 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN1449300) Images
Specificity This antibody reacts with Human EGF-R. It does not recognize human c-erbB2.
Purification Supernatant
Immunogen Recombinant human EGF-R cytoplasmic region (648-1186 aa)
Clone 6F1
Isotype IgG2b
Plasmids, Primers & others

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF antibody (ABIN1449300) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background The epidermal growth factor receptor (EGFR, ErbB-1) is a 170 kDa transmembrane glycoprotein that possesses intrinsic tyrosine kinase activity. There are several EGF family ligands including epidermal growth factor (EGF), amphiregulin (AR), heparin-binding epidermal growth factor (HB-EGF), epiregulin, betacellulin, transforming growth factor-a (TGF-a) and epigen, which can bind and activate the EGFR. Ligand binding facilitates dimerization of the EGFR, which activates downstream pathways known to be involved in cell growth, proliferation, differentiation and migration. Other members of the receptor tyrosine kinase family, which include EGFR, ErbB-2/HER-2, ErbB-3/HER-3 and ErbB-4/HER-4, are also known to play important roles in regulating a wide variety of cellular functions. Overexpression by gene amplification or mutation of these receptors correlates with the development and progression of several human cancers, as well as poor prognosis. Consequently, these receptors have been identified not only as a possible prognostic markers but also primarily a rational molecular target for an anticancer agents.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Gene ID 1950
NCBI Accession NP_001954
UniProt P01133
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF antibody (ABIN1449300) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF antibody (ABIN1449300) Images back to top
Reconstitution Prepare a stock solution by dissolving the lyophilized antibody in 1 mL of distilled water. After reconstitution, the protein concentration should be 1.1 mg/mL and the IgG concentration should be 100 μg/mL.
Buffer PBS ( pH 7.2), 0.09 % NaN3, 1 % Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted in aliquots at -20 °C.

References for anti-EGF antibody (ABIN1449300)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Background publications

Yamasaki, Tani, Yamamoto, Kitamura, Komada: "The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A." in: Molecular biology of the cell, Vol. 17, Issue 11, pp. 4876-87, 2006

Mizuno, Iura, Mukai, Yoshimori, Kitamura, Komada: "Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5163-74, 2005

Mizuno, Kawahata, Kato, Kitamura, Komada: "STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif." in: Molecular biology of the cell, Vol. 14, Issue 9, pp. 3675-89, 2003

Higuchi, Watanabe, Yamamoto: "Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of the Zn finger transcriptional activator Rst2p in fission yeast." in: Molecular and cellular biology, Vol. 22, Issue 1, pp. 1-11, 2001

Liu, Gong, Guo, Katagiri, Jiang, Wang, Johnson, Guroff: "The Wilms' tumor gene product WT1 mediates the down-regulation of the rat epidermal growth factor receptor by nerve growth factor in PC12 cells." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 5068-73, 2001

Hashimoto, Katayama, Kiyokawa, Ota, Kurata, Gotoh, Otsuka, Shibata, Matsuda: "Phosphorylation of CrkII adaptor protein at tyrosine 221 by epidermal growth factor receptor." in: The Journal of biological chemistry, Vol. 273, Issue 27, pp. 17186-91, 1998

Shibutani, Lazarovici, Johnson, Katagiri, Guroff: "Transcriptional down-regulation of epidermal growth factor receptors by nerve growth factor treatment of PC12 cells." in: The Journal of biological chemistry, Vol. 273, Issue 12, pp. 6878-84, 1998

Krueger, Daaka, Pitcher, Lefkowitz: "The role of sequestration in G protein-coupled receptor resensitization. Regulation of beta2-adrenergic receptor dephosphorylation by vesicular acidification." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 5-8, 1997

Lazarovici, Oshima, Shavit, Shibutani, Jiang, Monshipouri, Fink, Movsesyan, Guroff: "Down-regulation of epidermal growth factor receptors by nerve growth factor in PC12 cells is p140(trk)-, Ras-, and Src-dependent." in: The Journal of biological chemistry, Vol. 272, Issue 17, pp. 11026-34, 1997

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN1449300) back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) (AA 648-1186) antibody (ABIN1449300) anti-Epidermal Growth Factor (EGF) (AA 648-1186) antibody