anti-Human EGF antibody for Neutralization
Recommended EGF Antibody (supplied by: Log in to see )
- Antigen
-
Epidermal Growth Factor (EGF) Antibodies
show synonyms for this antigen- HOMG4
- URG
- AI790464
- CEGF
- epidermal growth factor
- pro-epidermal growth factor
- EGF
- egf
- CpipJ_CPIJ020278
- Egf
- Reactivity
-
Human
Alternatives - Host
-
Rabbit
Alternatives - Clonality
- Conjugate
-
This EGF antibody is un-conjugated
Alternatives - Application
-
ELISA, Neutralization (Neut), Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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Catalog No. ABIN465547
$ 493.17
Plus shipping costs $45.00
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ABIN223519 | Neut WB | Rabbit | IgG | Log in to see | Polyclonal | 1 | |||
| 1 | ABIN465856 | ELISA Neut WB | Biotin | Rabbit | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN242161 | Neut ELISA WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
| 1 | ABIN5506793 | Neut | Rabbit | IgG | AA 971-1023 | Log in to see | R001 | 0 | ||
| 1 | ABIN346557 | Neut ELISA WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
| 1 | ABIN2257864 | Neut ELISA WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN1983159 | FACS Neut WB | Mouse | IgG1 | AA 971-1023 | Log in to see | MM0255-4T32 | 0 | ||
| 1 | ABIN2260959 | Neut ELISA WB | Alkaline Phosphatase (AP) | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2260962 | Neut ELISA WB | FITC | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2260960 | Neut ELISA WB | APC | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2260961 | Neut ELISA WB | Biotin | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2260964 | Neut ELISA WB | PE | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2260963 | Neut ELISA WB | HRP | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2260958 | Neut ELISA WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN5688983 | ELISA Neut WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
| 1 | ABIN2139666 | Neut WB | Rabbit | IgG | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | Epidermal Growth Factor (EGF) Antibodies |
| Reactivity | Human Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This EGF antibody is un-conjugated Alternatives |
| Application |
ELISA, Neutralization (Neut), Western Blotting (WB)
Alternatives
|
| Supplier | Log in to see |
Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling Images |
|
| Specificity | Human EGF |
| Purification | Immunoaffinity purified |
| Immunogen |
E.coli derived recombinant Human EGF. Type of Immunogen: Cell extract |
| Plasmids, Primers & others | |
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling Images back to top |
|
| Antigen | |
| Alternative Name | EGF (EGF Antibody Abstract) |
| Background |
Name/Gene ID: EGF Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor |
| Gene ID | 1950 |
| UniProt | P01133 |
| Pathways | NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation |
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling Images back to top |
|
| Application Notes |
Approved: ELISA (0.5 - 2 μg/mL), Neut (0.25 - 0.35 μg/mL), WB Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL-0.25 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hEGF (25 pg/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (LS-C104809) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. |
| Comment |
Target Species of Antibody: Human |
| Restrictions | For Research Use only |
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details Images back to top |
|
| Format | Lyophilized |
| Reconstitution | Sterile water |
| Concentration | Lot specific |
| Buffer | Lyophilized from PBS, pH 7.2 |
| Handling Advice | Avoid freeze/thaw cycles. |
| Storage | 4 °C,-20 °C |
| Storage Comment |
Store Lyophilized at room temperature up to 1 month Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles. |
ImagesProduct Details anti-EGF Antibody Target Details EGF Application Details Handling back to top |
|
| Supplier Images |
anti-Epidermal Growth Factor (EGF) antibody
anti-Epidermal Growth Factor (EGF) antibody (Image 2)
anti-Epidermal Growth Factor (EGF) antibody (Image 3)
anti-Epidermal Growth Factor (EGF) antibody (Image 4)
anti-Epidermal Growth Factor (EGF) antibody (Image 5)
anti-Epidermal Growth Factor (EGF) antibody (Image 6)
anti-Epidermal Growth Factor (EGF) antibody (Image 7)
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