anti-Human EGF antibody for Western Blotting

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Reactivity
Human
382
186
109
20
17
9
4
2
2
1
1
1
Host
Mouse
370
148
32
26
6
3
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
57
29
23
11
11
9
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
418
282
159
101
54
33
30
30
27
27
23
16
14
7
7
5
4
3
2
1
1
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Catalog No. ABIN969091
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.05837 ABIN1724732 ICC ELISA WB Mouse IgG1 Log in to see 4-00E-11 2
22.55837 ABIN931410 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11 0
19.55837 ABIN3016522 IHC WB Rabbit Log in to see Polyclonal 0
19 ABIN465547 ELISA Neut WB Rabbit Log in to see Polyclonal 0
17.255764 ABIN966030 IF ELISA WB Mouse IgG1 Log in to see 9D7F11 4
17.255764 ABIN966031 IHC ELISA WB Mouse IgG1 Log in to see 4E11 4
16.55837 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
16.55837 ABIN634270 WB Rabbit Log in to see Polyclonal 0
16.55837 ABIN933589 ELISA WB Mouse IgG1 Log in to see KT57 0
13.802607 ABIN656805 IHC (p) WB Rabbit Ig Fraction AA 690-720, Center Log in to see Polyclonal 1
13.802607 ABIN1843510 IHC ELISA WB Mouse IgG1 Log in to see 0
13.802607 ABIN1843511 ELISA IF/ICC WB Mouse IgG1 Log in to see 0
12.755764 ABIN5539508 ELISA WB Goat Internal Region Log in to see Polyclonal 0
12.755764 ABIN4901429 ELISA IHC (p) WB Rabbit IgG AA 1000-1060 Log in to see Polyclonal 0
12.755764 ABIN4901430 ELISA WB Mouse IgG1 Log in to see 5E9-A10-A6 0
12.755764 ABIN4901431 ELISA WB Mouse IgG1 Log in to see 7C11-G10-H8 0
12.755764 ABIN4901428 ELISA IF WB Mouse IgG1 Log in to see 4E11 0
12.755764 ABIN4901427 ELISA IHC (p) WB Mouse IgG1 Log in to see 9D7F11 0
12.755764 ABIN5696014 WB Atto 488 Rabbit AA 670-770 Log in to see Polyclonal 0
12.755764 ABIN5696020 WB Atto 700 Rabbit AA 670-770 Log in to see Polyclonal 0

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(382), (186), (109), (20), (17), (9), (4), (2), (2), (1), (1), (1)
Host Mouse
(370), (148), (32), (26), (6), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(57), (29), (23), (11), (11), (9), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(418), (282), (159), (101), (54), (33), (30), (30), (27), (27), (23), (16), (14), (7), (7), (5), (4), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN969091) Images
Purification Ascites
Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Clone 9D7F11
Isotype IgG1
Plasmids, Primers & others

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF antibody (ABIN969091) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Molecular Weight 134 kDa
Gene ID 1950
HGNC 1950
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF antibody (ABIN969091) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF antibody (ABIN969091) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-EGF antibody (ABIN969091)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003

Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002

Background publications

Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN969091) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor (EGF) antibody (ABIN969091) Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung ...
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor (EGF) antibody (ABIN969091) Figure 2: Immunohistochemical analysis of paraffin-embedded human Uterus tissues usin...
Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) antibody (ABIN969091) Figure 3: Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HE...