anti-Pig (Porcine) EGF antibody for ELISA

Recommended EGF Antibody

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Epitope
AA 45-275
18
16
16
13
12
11
11
11
10
8
8
8
7
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Wild boar (Sus scrofa), Pig (Porcine)
388
170
95
20
16
9
3
2
2
2
1
1
Host
Rabbit
380
145
32
9
7
2
Clonality
Polyclonal
Conjugate
This EGF antibody is conjugated to Biotin
57
27
21
11
11
9
7
7
7
7
7
7
4
4
4
4
4
3
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
390
283
128
67
54
33
29
29
27
26
23
16
13
7
7
5
4
2
2
1
1
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Available images

Catalog No. ABIN1173139
$ 416.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN3201444 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 45-275 0

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 45-275
(18), (16), (16), (13), (12), (11), (11), (11), (10), (8), (8), (8), (7), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Wild boar (Sus scrofa), Pig (Porcine)
(388), (170), (95), (20), (16), (9), (3), (2), (2), (2), (1), (1)
Host Rabbit
(380), (145), (32), (9), (7), (2)
Clonality
Polyclonal
Conjugate This EGF antibody is conjugated to Biotin
(57), (27), (21), (11), (11), (9), (7), (7), (7), (7), (7), (7), (4), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(390), (283), (128), (67), (54), (33), (29), (29), (27), (26), (23), (16), (13), (7), (7), (5), (4), (2), (2), (1), (1)
Supplier

Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Sequence MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
Specificity It has been selected for its ability to recognize EGF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant EGF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin.
Isotype IgG
Plasmids, Primers & others

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant EGF (Ala45~His275) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) (AA 45-275) antibody (Biotin) (ABIN1173139) anti-Epidermal Growth Factor (EGF) (AA 45-275) antibody (Biotin)