anti-Rat (Rattus) EGF antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Epitope
AA 974-1026
25
18
17
16
12
12
11
11
10
9
8
8
7
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
389
188
109
20
17
9
4
2
2
2
1
1
Host
Rabbit
375
149
32
26
7
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
57
29
23
11
11
9
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
424
285
162
100
54
33
30
30
27
27
23
16
14
7
7
5
4
2
2
1
1
Options
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Catalog No. ABIN5518908
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.354353 ABIN1385702 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
13 ABIN3201446 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see 0
11.854353 ABIN498560 IHC (p) WB Rabbit Log in to see Polyclonal 0
5.3131986 ABIN446990 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 3
4 ABIN676643 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal 0
4 ABIN2692016 IHC (p) ELISA Rabbit IgG AA 974-1026 Log in to see Polyclonal 0
4 ABIN5957930 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2892928 IHC IHC (p) WB Rabbit Arg709 Log in to see Polyclonal 0
1 ABIN1414185 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN676652 IHC (p) HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN676645 IHC (p) Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1396308 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1385701 IF (p) IHC (p) WB Mouse IgG Log in to see Polyclonal 0
1 ABIN5647306 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
-13.041155 ABIN4307173 IHC IHC (p) WB Alexa Fluor 488 Rabbit Internal Region Log in to see Polyclonal 0
-13.041155 ABIN4307161 IHC IHC (p) WB Biotin Rabbit Internal Region Log in to see Polyclonal 0
-13.041155 ABIN4307163 IHC IHC (p) WB DyLight 405 Rabbit Internal Region Log in to see Polyclonal 0
-13.041155 ABIN4307162 IHC IHC (p) WB DyLight 350 Rabbit Internal Region Log in to see Polyclonal 0
-13.041155 ABIN4307154 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
-13.041155 ABIN4307165 IHC IHC (p) WB DyLight 488 Rabbit Internal Region Log in to see Polyclonal 0

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 974-1026
(25), (18), (17), (16), (12), (12), (11), (11), (10), (9), (8), (8), (7), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(389), (188), (109), (20), (17), (9), (4), (2), (2), (2), (1), (1)
Host Rabbit
(375), (149), (32), (26), (7), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(57), (29), (23), (11), (11), (9), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(424), (285), (162), (100), (54), (33), (30), (30), (27), (27), (23), (16), (14), (7), (7), (5), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN5518908) Images
Purpose Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(Egf) detection. Tested with WB, IHC-P, ELISA in Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(Egf) detection. Tested with WB, IHC-P, ELISA in Rat.
Gene Name: epidermal growth factor
Protein Name: Pro-epidermal growth factor
Purification Immunogen affinity purified.
Immunogen E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.
Isotype IgG
Plasmids, Primers & others

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF antibody (ABIN5518908) Images back to top
Antigen
Alternative Name Egf (EGF Antibody Abstract)
Background EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms: Pro-epidermal growth factor, EGF, Epidermal growth factor, Egf
Gene ID 25313
UniProt P07522
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF antibody (ABIN5518908) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF antibody (ABIN5518908) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-EGF antibody (ABIN5518908)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Wang, Li, Fu, Li, Yang, Zhang, Zhu, Yang, Gu, Xing, Zhang: "Exemestane Attenuates Hepatic Fibrosis in Rats by Inhibiting Activation of Hepatic Stellate Cells and Promoting the Secretion of Interleukin 10." in: Journal of immunology research, Vol. 2017, pp. 3072745, 2018

Messini: "[Therapy of total arrhythmia due to atrial fibrillation and flutter]." in: La Clinica terapeutica, Vol. 57, Issue 2, pp. 109-28, 1971

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN5518908) back to top
Supplier Images
 image for anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody (ABIN5518908) Western blot analysis of EGF using anti-EGF antibody . Electrophoresis was performed...
 image for anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody (ABIN5518908) IHC analysis of EGF using anti-EGF antibody . EGF was detected in paraffin-embedded s...
 image for anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody (ABIN5518908) IHC analysis of EGF using anti-EGF antibody . EGF was detected in paraffin-embedded s...