anti-Rat (Rattus) EGF antibody for Neutralization
Recommended EGF Antibody (supplied by: Log in to see )
- Antigen
-
Epidermal Growth Factor (EGF) Antibodies
show synonyms for this antigen- HOMG4
- URG
- AI790464
- CEGF
- epidermal growth factor
- pro-epidermal growth factor
- EGF
- egf
- CpipJ_CPIJ020278
- Egf
- Reactivity
-
Rat (Rattus)
Alternatives - Host
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Rabbit
Alternatives - Clonality
- Conjugate
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This EGF antibody is un-conjugated
Alternatives - Application
-
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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Catalog No. ABIN465662
$ 493.17
Plus shipping costs $45.00
Plus shipping costs $45.00
| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ABIN465685 | ELISA IHC Neut WB | Biotin | Rabbit | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2257874 | Neut IHC ELISA WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2257897 | Neut IHC ELISA WB | APC | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2257896 | Neut IHC ELISA WB | Alkaline Phosphatase (AP) | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2257899 | Neut IHC ELISA WB | FITC | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2257900 | Neut IHC ELISA WB | HRP | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2257898 | Neut IHC ELISA WB | Biotin | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2257901 | Neut IHC ELISA WB | PE | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN5690467 | ELISA Neut WB | Rabbit | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | Epidermal Growth Factor (EGF) Antibodies |
| Reactivity | Rat (Rattus) Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This EGF antibody is un-conjugated Alternatives |
| Application |
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
Alternatives
|
| Supplier | Log in to see |
Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling Images |
|
| Specificity | Rat EGF |
| Purification | Immunoaffinity purified |
| Immunogen |
E.coli derived recombinant Rat EGF. Type of Immunogen: Cell extract |
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling Images back to top |
|
| Antigen | |
| Alternative Name | EGF (EGF Antibody Abstract) |
| Background |
Name/Gene ID: EGF Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor |
| Gene ID | 1950 |
| UniProt | P01133 |
| Pathways | NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation |
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling Images back to top |
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| Application Notes |
Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (0.1 μg/mL), WB Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (mammillary nuclei) tissue. The primary antibody was incubated at 1 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat EGF (0.1 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF (LS-C104723) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat EGF. Western Blot: To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details Images back to top |
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| Format | Lyophilized |
| Reconstitution | Sterile water |
| Concentration | Lot specific |
| Buffer | Lyophilized from PBS, pH 7.2 |
| Handling Advice | Avoid freeze/thaw cycles. |
| Storage | 4 °C,-20 °C |
| Storage Comment |
Store Lyophilized at room temperature up to 1 month Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles. |
ImagesProduct Details anti-EGF Antibody Target Details EGF Application Details Handling back to top |
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| Supplier Images |
anti-Epidermal Growth Factor (EGF) antibody
anti-Epidermal Growth Factor (EGF) antibody (Image 2)
anti-Epidermal Growth Factor (EGF) antibody (Image 3)
anti-Epidermal Growth Factor (EGF) antibody (Image 4)
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