anti-Rat (Rattus) EGF antibody for Neutralization

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Reactivity
Rat (Rattus)
387
187
108
20
17
9
4
2
2
1
1
1
Host
Rabbit
373
149
32
26
7
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
57
29
23
11
11
9
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Neutralization (Neut), Western Blotting (WB)
423
285
161
101
54
32
30
30
27
27
23
16
14
7
7
5
4
2
2
1
1
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Catalog No. ABIN465662
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465685 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN2257874 Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2257896 Neut IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2257897 Neut IHC ELISA WB APC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2257899 Neut IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2257900 Neut IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN2257898 Neut IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN2257901 Neut IHC ELISA WB PE Rabbit IgG Log in to see Polyclonal 0
1 ABIN5690467 ELISA Neut WB Rabbit Log in to see Polyclonal 0

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Rat (Rattus)
(387), (187), (108), (20), (17), (9), (4), (2), (2), (1), (1), (1)
Host Rabbit
(373), (149), (32), (26), (7), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(57), (29), (23), (11), (11), (9), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Neutralization (Neut), Western Blotting (WB)
(423), (285), (161), (101), (54), (32), (30), (30), (27), (27), (23), (16), (14), (7), (7), (5), (4), (2), (2), (1), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity Rat EGF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Rat EGF.

Type of Immunogen: Cell extract
Plasmids, Primers & others

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Gene ID 1950
UniProt P01133
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (mammillary nuclei) tissue. The primary antibody was incubated at 1 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat EGF (0.1 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF (LS-C104723) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat EGF. Western Blot: To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Comment

Target Species of Antibody: Rat
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Lyophilized
Reconstitution Sterile water
Concentration Lot specific
Buffer Lyophilized from PBS,  pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
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image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465662) anti-Epidermal Growth Factor (EGF) antibody (Image 3)
image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465662) anti-Epidermal Growth Factor (EGF) antibody (Image 4)