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|Antigen||Epidermal Growth Factor (EGF) Antibodies|
|Epitope||AA 45-275 Alternatives|
|Reactivity||Wild boar (Sus scrofa), Pig (Porcine) Alternatives|
|Conjugate||This EGF antibody is conjugated to Biotin Alternatives|
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Product Details anti-EGF AntibodyTarget Details EGF Application Details Handling Images
|Sequence||MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH|
|Specificity||It has been selected for its ability to recognize EGF in immunohistochemical staining and Western blotting.|
Recombinant EGF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin.
|Plasmids, Primers & others|
Target Details EGFProduct Details anti-EGF Antibody Application Details Handling Images back to top
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation|
Application DetailsProduct Details anti-EGF Antibody Target Details EGF Handling Images back to top
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Content: The quality control contains recombinant EGF (Ala45~His275) disposed in loading buffer.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGF Antibody Target Details EGF Application Details Images back to top
|Buffer||Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid repeated freeze/thaw cycles|
|Storage Comment||Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.|
|Expiry Date||12 months|
ImagesProduct Details anti-EGF Antibody Target Details EGF Application Details Handling back to top