anti-Human EGFR antibody for Flow Cytometry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • LOC5564544
Epitope
pTyr1173
151
128
67
63
58
56
56
54
53
52
48
48
43
42
39
36
33
31
30
29
27
25
25
25
22
19
18
18
18
18
16
16
16
16
16
15
15
15
15
15
14
14
13
11
9
9
8
8
7
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2428
851
684
29
24
18
17
15
15
8
3
2
2
1
1
1
1
Host
Rabbit
1467
859
79
44
9
5
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
104
82
73
65
56
54
54
32
32
28
28
28
28
28
28
28
26
25
24
24
21
21
21
21
21
21
20
18
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
1484
684
629
541
515
272
264
225
125
90
65
56
42
24
19
14
12
11
11
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN4900628
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.98401 ABIN1724825 FACS IHC ELISA WB Mouse IgG1 AA 693-893, Mutant Log in to see 5G9B5 2
13.98401 ABIN1724824 FACS IHC ELISA WB Mouse IgG1 AA 693-893, Mutant Log in to see 5G9B5 2
13.98401 ABIN4896907 FACS PE Rat IgG2a AA 25-645 Log in to see 423103 1
13.98401 ABIN4899924 ELISA (Detection) FACS WB Biotin Goat IgG AA 25-645 Log in to see Polyclonal 4
13.98401 ABIN4900626 CyTOF FACS WB Mouse IgG2a pTyr1068 Log in to see 338324 2
13.98401 ABIN1724889 FACS ELISA WB Mouse IgG2b AA 693-893 Log in to see 5E10D3 2
13.98401 ABIN1724890 FACS IHC ELISA WB Mouse IgG1 AA 693-893 Log in to see 7A6F12 2
13.98401 ABIN4899925 CyTOF ELISA (Capture) FACS ICC IHC IP WB Goat IgG AA 25-645 Log in to see Polyclonal 6
13.98401 ABIN4900636 CyTOF FACS ICC IHC WB Rabbit IgG pTyr845 Log in to see Polyclonal 2
13.98401 ABIN651628 FACS IHC (p) WB Rabbit Ig Fraction AA 1004-1033, C-Term Log in to see Polyclonal 1
13.98401 ABIN1995316 FACS FITC Mouse IgG1 AA 1-645 Log in to see 08 0
13.98401 ABIN4896905 FACS Fluorescein Rat IgG2a AA 25-645 Log in to see 423103 2
13.98401 ABIN1995318 FACS PE Mouse IgG1 AA 1-645 Log in to see 08 0
13.98401 ABIN1995326 FACS PE Rabbit IgG AA 1-645 Log in to see E01 0
13.98401 ABIN1995324 FACS FITC Rabbit IgG AA 1-645 Log in to see E01 0
13.98401 ABIN153570 FACS IHC (fro) IHC (p) IP IHC Rat IgG2a Log in to see ICR10 1
13.98401 ABIN4896908 FACS Alexa Fluor 647 Rat IgG2a AA 25-645 Log in to see 423103 0
13.98401 ABIN4898092 FACS Fluorescein Mouse IgG2a pTyr1068 Log in to see 338324 1
13.98401 ABIN153598 FACS FITC Rat IgG2a Log in to see ICR10 1
13.98401 ABIN4307221 FACS IF IHC (p) Mouse IgG2a kappa Log in to see GFR450 0

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1173
(151), (128), (67), (63), (58), (56), (56), (54), (53), (52), (48), (48), (43), (42), (39), (36), (33), (31), (30), (29), (27), (25), (25), (25), (22), (19), (18), (18), (18), (18), (16), (16), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (9), (9), (8), (8), (7), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2428), (851), (684), (29), (24), (18), (17), (15), (15), (8), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(1467), (859), (79), (44), (9), (5), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(104), (82), (73), (65), (56), (54), (54), (32), (32), (28), (28), (28), (28), (28), (28), (28), (26), (25), (24), (24), (21), (21), (21), (21), (21), (21), (20), (18), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(1484), (684), (629), (541), (515), (272), (264), (225), (125), (90), (65), (56), (42), (24), (19), (14), (12), (11), (11), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4900628) Images
Specificity Detects human EGFR when phosphorylated at Y1173 in Western blots.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Phosphopeptide containing human EGFR Y1173 site
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN4900628) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Alternative Names: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene ID 1956
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN4900628) Images back to top
Application Notes Western Blot 0.2 μg/mL
Simple Western 2 μg/mL
Immunohistochemistry 5-15 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN4900628) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EGFR antibody (ABIN4900628)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Kefaloyianni, Muthu, Kaeppler, Sun, Sabbisetti, Chalaris, Rose-John, Wong, Sagi, Waikar, Rennke, Humphreys, Bonventre, Herrlich: "ADAM17 substrate release in proximal tubule drives kidney fibrosis." in: JCI insight, Vol. 1, Issue 13, 2016 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Qi, Duhachek-Muggy, Li, Zolkiewska: "Phenotypic diversity of breast cancer-related mutations in metalloproteinase-disintegrin ADAM12." in: PLoS ONE, Vol. 9, Issue 3, pp. e92536, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Weissenbacher, Vrekoussis, Roeder, Makrigiannakis, Mayr, Ditsch, Friese, Jeschke, Dian: "Analysis of Epithelial Growth Factor-Receptor (EGFR) Phosphorylation in Uterine Smooth Muscle Tumors: Correlation to Mucin-1 and Galectin-3 Expression." in: International journal of molecular sciences, Vol. 14, Issue 3, pp. 4783-92, 2013 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Xue, Campbell, Jackson: "Protein C is an autocrine growth factor for human skin keratinocytes." in: The Journal of biological chemistry, Vol. 282, Issue 18, pp. 13610-6, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Kaneko, Kubo, Hata, Yamaguchi, Yamashita: "Repulsion of cerebellar granule neurons by chondroitin sulfate proteoglycans is mediated by MAPK pathway." in: Neuroscience letters, Vol. 423, Issue 1, pp. 62-7, 2007 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4900628) back to top
Supplier Images
Simple Western (SimWes) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN4900628) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody
Flow Cytometry (FACS) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN4900628) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (Image 2)
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN4900628) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN4900628) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (Image 4)
Immunocytochemistry (ICC) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN4900628) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (Image 5)
Simple Western (SimWes) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN4900628) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (Image 6)