anti-Human EGFR antibody for Indirect Immunofluorescence Assay

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pTyr1173
147
134
64
64
60
58
57
54
54
53
49
49
43
38
36
33
32
30
29
29
27
26
26
25
24
22
19
18
18
17
16
16
16
15
15
15
15
15
14
14
13
11
10
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2444
866
700
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1462
867
92
44
11
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
103
80
71
64
58
56
34
34
28
28
28
28
28
28
28
27
27
26
26
26
24
21
21
21
21
21
20
19
18
4
2
2
1
Application
Indirect Immunofluorescence Assay (IFA), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
1451
702
614
556
511
272
260
229
127
84
66
56
45
24
19
14
12
11
7
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN235075
$ 862.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN235074 IFA ELISA WB Mouse IgG1 pSer1047 Log in to see 1H9

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1173
(147), (134), (64), (64), (60), (58), (57), (54), (54), (53), (49), (49), (43), (38), (36), (33), (32), (30), (29), (29), (27), (26), (26), (25), (24), (22), (19), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (10), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2444), (866), (700), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1462), (867), (92), (44), (11), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(103), (80), (71), (64), (58), (56), (34), (34), (28), (28), (28), (28), (28), (28), (28), (27), (27), (26), (26), (26), (24), (21), (21), (21), (21), (21), (20), (19), (18), (4), (2), (2), (1)
Application Indirect Immunofluorescence Assay (IFA), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(1451), (702), (614), (556), (511), (272), (260), (229), (127), (84), (66), (56), (45), (24), (19), (14), (12), (11), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity Binding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues, Y1173 being the major autophosphorylation site. Clone 9H2 specifically interacts with the 1170 - NAEpYLRV motif corresponding to the major autophosphorylation site of human EGFR. The antibody does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. This phosphorylation site-specific monoclonal antibody allows the detection and quantification of activated EGFR in crude cell extracts in Immunoblot, ELISA and Biosensor assay formats without the use of P32.
Cross-Reactivity (Details) Reacts with Human, mouse, and dog.
Characteristics Cell lysate of pervanadate-treated HepG2 cells, Cell culture
Purification Thiophilic adsorption and size exclusion Chromatography. Cell culture
Immunogen Phosphopeptide conjugated to KLH
Clone 9H2
Isotype IgG1
Fragment Chain kappa

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Research Area Growth Factors, Hormones, Cancer
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Immunoblotting: 0.5 µg/mL for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5 % (w/v) casein, 5 % (w/v) PEG 4000, 5 % (w/v) Polyvinylpyrrolidone (PVP), 0.5 % Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (20.000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5- g/mL K67902M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Immunoprecipitation: 1-10ug per 106 vanadate treated A431 cells. Immunocytochemistry: 1-10 µg/mL ELISA: 0.05 µg/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with 1 mL water for 15 minutes at room temperature.
Buffer Lyophilized from PBS, PEG and Sucrose, 0.09 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C/-80 °C
Storage Comment Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored at 2-0 °C for up to 3 months.

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
 image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN235075) Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or perv...