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|Antigen||Interleukin 1 alpha (IL1A) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This IL1A antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-IL1A AntibodyTarget Details IL1A Application Details Handling Images
E.coli derived recombinant Rat IL-1alpha.
Type of Immunogen: Cell extract
Target Details IL1AProduct Details anti-IL1A Antibody Application Details Handling Images back to top
|Alternative Name||IL1A / IL1 Alpha (IL1A Antibody Abstract)|
Name/Gene ID: IL1A
Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha
|Pathways||NF-kappaB Signaling, Autophagy|
Application DetailsProduct Details anti-IL1A Antibody Target Details IL1A Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (11 - 17 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 1 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IL-1alpha (50 pg/mL), a concentration of 0.011-0.017 μg/mL of this antibody is required. ELISA: To detect Rat IL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1alpha (LS-C104576) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1alpha. Western Blot: To detect Rat IL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1A Antibody Target Details IL1A Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-IL1A Antibody Target Details IL1A Application Details Handling back to top